Poly(oxy-1,2-ethanediyl), α-[2-(dide- cylmethylammonio)ethyl]- .omega.- hydroxy-, propanoate (salt) (Bardap 26)

Sediment toxicity

Link to relevant study record(s)

Reference

Endpoint:

sediment toxicity: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17/11/1993 - 15/12/1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: American Society for Testing Materials (1992) ASTM Document No E 1383-93

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: U.S. EPA-600/3-75-009

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: American Society for Testing Materials (1992) ASTM Document No E 729-88a

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Standard Methods for the Examination of Water and Wastewater, American Public Health Association, Washington DC, 17th edition 1995

Deviations:

no

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No information provided.

Analytical monitoring:

yes

Details on sampling:

Duplicate analytical replicates were taken on days 0, 14, and 28 for analysis of 14-C residues in the water column, the interstitial water, and the sediment. Temperature, dissolved oxygen and pH were measured twice weekly

Vehicle:

not specified

Details on sediment and application:

The sediment was from an agriculture pond, with a pH of 4.9. The test was conducted in 2 l glass beakers containing approximately 300 ml of sediment and 1500 ml of hard blended water. In addition to the six biological replicates, six analytical (abiotic) replicates of each concentration were prepared to provide analytical samples of water column, interstitial water (by centrifugation), and sediment. No test organisms were added to these replicates. A sediment control was also used.

Test organisms (species):

Chironomus dilutus (previous name: Chironomus tentans)

Details on test organisms:

-Test organism: Chironomus tentans
-Developmental stage: Second instar
- Source: ABC Laboratories’ in-house culture
- Lot number: CT-23593

Study type:

laboratory study

Test type:

static

Water media type:

freshwater

Type of sediment:

natural sediment

Limit test:

no

Duration:

28 d

Exposure phase:

total exposure duration

Post exposure observation period:

Observations were recorded at intervals up to 28 days following the start of exposure.

Hardness:

146 mg/l as CaCO3

Test temperature:

21-22°C

pH:

Water column pH: 6.9-8.3
Soil pH: 4.9

Dissolved oxygen:

3.3 - 8.1 mg/l

Salinity:

Conductivity: 340 µMhos/cm

Ammonia:

No information provided.

Nominal and measured concentrations:

Initial test concentrations: 126, 249, 501, 1000 and 1999 mg/kg
Actual test concentrations: 150, 260, 530, 1000 and 2200 mg/kg

Details on test conditions:

Six replicate groups of 10 midge larvae each were exposed to each of the test concentrations. The test was conducted in 2 l glass beakers containing approximately 300 ml of sediment and 1500 ml of hard blended water. In addition to the six biological replicates, six analytical (abiotic) replicates of each concentration were prepared to provide analytical samples of water column, interstitial water (by centrifugation), and sediment. No test organisms were added to these replicates. Duplicate analytical replicates were exposed on days 0, 14 and 28 for analysis of 14-C residues in each of the three exposure matrices.

Reference substance (positive control):

not specified

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

530 mg/kg sediment dw

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: growth and emergence success

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

530 mg/kg sediment dw

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: growth and emergence success

Key result

Duration:

28 d

Dose descriptor:

LC50

Effect conc.:

2 085 mg/kg sediment dw

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: growth and emergence success

Details on results:

The results of this study indicated that survival of Chironomus tentans, including survival to successful emergence, was reduced as a result of exposure to the test substance at certain concentrations. Adverse effects on growth, as determined by day 14 larval weights, and time to emergence were also observed as a result of exposure to sediment concentrations above 100 mg/kg. A 14-day EC50 based on total adverse effects was calculated to be 1287 mg/kg. The 28-day LC50 was calculated to be 2085 mg/kg, while the NOEC for the study was 530 mg/kg

Results with reference substance (positive control):

No information provided.

Reported statistics and error estimates:

Computerised LC50 program Stephan et al.

Table 1. LC50/EC50, NOEC/LOEC and MATC

 

	Day 14	Day 28
LC50 mg/kg	>1000	2085
(95% confidence limits mg/kg)	-	(1000-2200)
EC50 mg/kg	1287	2085
(95% confidence limits mg/kg)	(1137-1483)	(1000-2000)
NOEC (mg/kg)	530^1	530^2
LOEC (mg/kg)	1000^1	1000^2
MATC (mg/kg)	728^1	728^2

¹ - Based on Growth

² - Based on Emergence

Validity criteria fulfilled:

yes

Conclusions:

Based on the results of this study, the test substance was found to have adverse effects on the test organisms when tested under the conditions of this study.The 14-day and 28-day NOEC were found to be 530 mg/kg, based on the growth and emergence success. The 28-day LC50 was 2085 mg/kg.
Survival of test organisms, including survival to successful emergence, was reduced as a result of exposure to the highest sediment concentration of the test substance.
Adverse effects on growth as determined by day 14 larval weights and time of emergence were observed as a result of exposure to sediment concentrations above 1000 mg/kg.
Although a 14-day LC50 could not be determined due to insufficient mortality, the 14-day EC50 based on total adverse effects was found to be 1287 mg/kg.

Executive summary:

In a study conducted in accordance with the ASTM Document No E 1383-93. American Society for Testing Materials (1992),  Methods for Acute Toxicity Test with Fish, Macroinvertebrates and Amphibians, U.S. EPA-600/3-75-009 Standard Methods for Conducting Basic Acute Toxicity Test with Fish , Macroinvertebrates and Amphibians, ASTM Document No E 729-88a American Society for Testing Materials (1992), Standard Methods for the Examination of Water and Wastewater, American Public Health Association, Washington DC, 17^thedition, Chironomus tentans were exposed the test material, Bardac 2280 with radiolabelled Didecyldimethylammonium Chloride, at concentrations of 126, 249, 501, 1000 and 1999 mg/kg (actual test concentrations: 150, 260, 530, 1000 and 2200 mg/kg) for a period of 28 days. The study was conducted in 2 L glass beakers containing approximately 300 ml of sediment and 1500 ml of hard blended water. Six replicate groups of 10 midge larvae each were exposed to each of the test concentrations. Six analytical (abiotic) replicates of each concentration were prepared to provide analytical samples of water column, interstitial water (by centrifugation), and sediment. Survival and growth were evaluated on day 14. Survival and emergence success were evaluated after 28 days of exposure.

The 14-day and 28-day NOEC were found to be 530 mg/kg, based on the growth and emergence success. The 28-day LC₅₀ was 2085 mg/kg. Based on the results of this study, the test substance was found to have adverse effects on the test organisms when tested under the conditions of this study.

Description of key information

One key study is available to assess the sediment toxicity of Bardac 2280.  

Key value for chemical safety assessment

EC50 or LC50 for freshwater sediment:

2 085 mg/kg sediment dw

EC10, LC10 or NOEC for freshwater sediment:

530 mg/kg sediment dw

Additional information

In a study conducted in accordance with the ASTM Document No E 1383-93. American Society for Testing Materials (1992),  Methods for Acute Toxicity Test with Fish, Macroinvertebrates and Amphibians, U.S. EPA-600/3-75-009 Standard Methods for Conducting Basic Acute Toxicity Test with Fish , Macroinvertebrates and Amphibians, ASTM Document No E 729-88a American Society for Testing Materials (1992), Standard Methods for the Examination of Water and Wastewater, American Public Health Association, Washington DC, 17th edition, Chironomus tentans were exposed the test material, Bardac 2280 with radiolabelled Didecyldimethylammonium Chloride, at concentrations of 126, 249, 501, 1000 and 1999 mg/kg (actual test concentrations: 150, 260, 530, 1000 and 2200 mg/kg) for a period of 28 days. The study was conducted in 2 L glass beakers containing approximately 300 ml of sediment and 1500 ml of hard blended water. Six replicate groups of 10 midge larvae each were exposed to each of the test concentrations. Six analytical (abiotic) replicates of each concentration were prepared to provide analytical samples of water column, interstitial water (by centrifugation), and sediment. Survival and growth were evaluated on day 14. Survival and emergence success were evaluated after 28 days of exposure.

The 14-day and 28-day NOEC were found to be 530 mg/kg, based on the growth and emergence success. The 28-day LC₅₀was 2085 mg/kg. The results indicate that under the conditions of this study DDAC caused adverse effects in the test organisms.