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EC number: 604-112-6 | CAS number: 139147-73-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-04-18 until 1997-08-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD test under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Silane CP 2
- Physical state: liquid
- Analytical purity: 96.3% (GC)
- Impurities (identity and concentrations): as stated in substance data
- Composition of test material, percentage of components: as stated in substance data
- Lot/batch No.: 10466 QB
- Expiration date of the lot/batch: 1997-12-01
- Stability under test conditions: reaction to hydrolysis product
- Storage condition of test material: in original container, at room temperature, in the dark
Sampling and analysis
- Analytical monitoring:
- not required
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Different test substance amounts were weighed by means of a Hamilton syringe and injected into 1000 ml brown glass flasks, filled with
284 ml of deionized water and stirred by magnetic stirrers for 30 minutes.
- Controls: inoculum control; 3,5-Dichlorophenol was used a positive control
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: domestic waste water treatment plant (ARA Ergolz II, Füllinsdorf/Switzerland)
- Method of cultivation: sludge was washed by centrifugation, the supernatant liquid phase was decanted, the solid material resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet sludge to its dry weight determined. Based on this ratio calculated aliquots of washed sludge suspension were made up with Soerensen buffer (pH 7), to obtain a concentration equivalent to 2 g dry weight basis per litre. To this mixture, 50 ml synthetic sewage feed per litre was added daily starting four days prior to use, and the sludge was kept at room temperature under continous aeration until use.
- Preparation of inoculum for exposure: Immediately before use, the dry weight of the activated sludge was determined to be 5.2 g/L, and diluted with tap water to a dry weight of 4 g/L. The pH was adjusted to 7.8 with 0.5 mol/L sulfuric acid.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not applicable
Test conditions
- Test temperature:
- 22 °C
- pH:
- 8.3 - 8.5
- Dissolved oxygen:
- test substance: start: 4.0 - 4.8 mg O2/L; end: 6.6 - 6.7 mg O2/L
control: start: 4.6 - 5.3 mg O2/L; end: 6.7 - 7.0 mg O2/L
positve control: 4.7 - 5.7 mg O2/L; end: 6.5 - 8.7 mg O2/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml brown glass flasks
- Aeration: immediately after filling the flasks aeration was started using compressed air (flow rate: approx. 1 L/minute)
- Test concentrations: positive control: 100, 32, 10, 3.2 mg/L; test substance: 100, 50, 24, 12, 6 mg/L
- Biomass loading rate: 1.6 g/L during the test
- Reference substance (positive control):
- yes
- Remarks:
- Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No inhibitory effect on the respiratory rate of activated sludge up to a highest test concentration of 100 mg/L has been observed.
- Executive summary:
The inhibitory effect of Silane CP 2 on the respiratory rate of aerobic waste water microorganisms of activated sludge was investigated in a 3-hour respiratory test in accordance with OECD Guideline 209. This study examined five test substance concentrations (6 to 100 mg/L), three inoculum controls and four concentrations of the positve control 3,5 -Dichlorophenol (3.2 to 100 mg/L). The respiratory rate of the activated sludge was not inhibited by the test substance at all test concentrations. The 3 -hour EC50 and EC20 are clearly higher than 100 mg/L (nominal).
The 3 -hour EC50 for the positive control was found to be 19.6 mg/L and was within the range of 5 - 30 mg/L recommended by the test guideline.
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