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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April - May 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
4.2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.25 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Validity criteria fulfilled:
yes
Conclusions:
The test substance had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus. The ErC50 (growth rate) was 4.2 mg/L on the basis of measured concentrations (TOC). The NOEC was 0.25 mg/L, the LOEC was 0.5 mg/L
Executive summary:

Z-Triamine-dighydrochloride was tested in an algal growth inhibition test. For the preparation of the test solutions 125 mg/L were dissolved in demineralized water and ultrasonified for approximately 30 min. The resulting stock solution was further diluted 1:8 to 1:400 giving test concentrations of 0.25 and 12.5 mg/L. The algae were exposed to each concentration in triplicate. Six vessels were prepared for the control. The algae were incubated under standardized conditions (continuous light, controlled temperature) in an incubator shaker apparatus. As a parameter for the growth of the algae population, the algae cells were measured with a coulter counter. The growth rate was calculated on the basis of the cell counts.The calculated growth rate of each concentration was compared to those of the controls, and the inhibition was calculated.

The growth rate was inhibited in a concentration-dependent manner. This was considered to be a compound related effect. The NOEC for growth rate inhibition was 0.25 mg/L, the LOEC was 0.5 mg/L and the ErC50 was 4.2 mg/L. .

Description of key information

The test substance had a concentration-depended inhibitory effect on the growth of Desmodesmus subspicatus. The ErC50 (growth rate) was 4.2 mg/L on the basis of measured concentrations (TOC). The NOEC was 0.25 mg/L, the LOEC was 0.5 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
4.2 mg/L

Additional information