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EC number: 923-035-1 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27.05.-24.06.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-Ethoxy-2,3-difluor-4-[4-(trans-4-butylcyclohexyl)-1-cyclohexen-1-yl]-benzene
- EC Number:
- 923-035-1
- Molecular formula:
- C24 H34 F2 O
- IUPAC Name:
- 1-Ethoxy-2,3-difluor-4-[4-(trans-4-butylcyclohexyl)-1-cyclohexen-1-yl]-benzene
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Wuzhong district urban area sewageTreatment Plant, treating predominantly domestic sewage
- Preparation of inoculum for exposure: Sludge was washed and centrifuged. Washing procedure repeated 3 times.
- Concentration of sludge: 4 g/L dw
- Initial cell/biomass concentration: 30 mg(L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 36 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: based salt medium
- Test temperature: 22 +- 1°C
- pH: 6-8.5
TEST SYSTEM
- Culturing apparatus: glass bottles 500 mL
- Number of culture flasks/concentration: 2
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: 1 flask
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 1
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 84 % in 14 days
Any other information on results incl. tables
Inoculum blank: O2 uptake 26.8 mg/L in 28 d
Toxicity control: 39 % in 14 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The ready biodegradabilityh of the test item was determined in a study according to OECD 301F (Manometric Respirometry Test). The biodegradation was determined to be <1% after 28 d. Biodegradation of the reference substance and the results of the controls were within the validity criteria. Therefore, the test item has to be considered as not readily biodegradable under the conditions of this test.
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