.beta.-D-Ribofuranose, 5-deoxy-, 1,2,3-triacetate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

other information

Study period:

August 27,2001 to December 19,2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Acetylfuranoside was investigated for its ready biodegradability in a "28-Day DOC Die-Away Test" according to EU Commission Directive 92/69 EEC, C.4-A (1992) and OECD Guideline for Testing of Chemicals, No. 301 A (1992).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Acetylfuranoside was investigated for its ready biodegradability in a "28-Day DOC Die-Away Test" according to EU Commission Directive 92/69 EEC, C.4-A (1992) and OECD Guideline for Testing of Chemicals, No. 301 A (1992).

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Acetylfuranoside

IUPAC Name:

Acetylfuranoside

Details on test material:

Expiration date: June 08,2002
Solubility in water: Equal or greather than 100 mg/L in test water (determined at RCC (non-GLP))
Aggregate state / physical form at room temperature: Solid / powder
Color: White
Storage conditions: Refrigerator, at approx. 4 "C under N2 (for quality reasons).

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

other: The test media were continuously stirred by magnetic stirrers.

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Remarks on exposure duration:

In accordance with the guidelines the test was ended before 28 days since Acetylfuranoside was completely biodegraded within approximately 10 days and the biodegradation curves of the test and the reference item had reached a plateau over 3 determinations

Results and discussion

Any other information on results incl. tables

DEGRADATION OF THE TEST ITEM

 

In the test flasks containing the test item and inoculum the mean concentration of DOC (dissolved organic carbon) rapidly decreased from initially 28 mg/L at Day 0 to 12 mg/L at Day 3. After approximately 7-10 days the test item was completely biodegraded (DOC values around 1.0 mg/L). An approximate 60% removal of DOC was already reached within 3 days of exposure.

 

Thus Acetylfuranoside can be stated as readily biodegradable since the pass level for ready biodegradability (70% removal of DOC in a 10-day window within a 28-day period) was reached.

.

Abiotic control:

In the abiotic control containing the test item and poisoned medium, the DOC concentration was 30 mg/L over the whole exposure period of 14 days. Thus, no abiotic degradation occurred under the test conditions.

 

DEGRADATION OF THE REFERENCE ITEM

In the procedure controls containing the reference item sodium benzoate and activated sludge (inoculum), sodium benzoate was completely biodegraded within 7 days of exposure, confirming the suitability of the activated sludge.

 

DEGRADATION IN THE TOXICITY CONTROL

In the toxicity control containing the test item (corresponding to 50% of total DOC), the reference item (corresponding to 50% of total DOC) and inoculum, the initial DOC concentration of 58 mg/L measured on Day 0 decreased by 102% within 14 days of exposure. Thus, according to the test guidelines the test item can be assumed not to be inhibitory to activated sludge because degradation was>35%within14days.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Acetylfuranoside can be stated as readily biodegradable since the pass level for ready biodegradability (70% removal of DOC in a 10-day window within a 28-day period) was reached.
According to the test guidelines the test item can be assumed to be not inhibitory to activated sludge because degradation was >35% within 14 days.

Executive summary:

Acetylfuranoside was investigated for its ready biodegradability in a "28-Day DOC Die-Away Test" according to EU Commission Directive 92/69 EEC, C.4-A (1992) and OECD Guideline for Testing of Chemicals,No.301 A (1992). In accordance with the guidelines the test was ended before 28 days since Acetylfuranoside was completely biodegraded within approximately 10 days and the biodegradation curves of the test and the reference item had reached a plateau over three determinations.

In the test flasks containing the test item Acetylfuranoside and inoculum the mean concentration of dissolved organic carbon (DOC) rapidly decreased within 3 days of exposure by approximately 60%. After approximately 10 days the test item was completely biodegraded.

Thus Acetylfuranoside can be stated as readily biodegradable since the pass level for ready biodegradability (70% removal of DOC in a 10-day window within a 28-day period) was reached.

In the abiotic control containing the test item and poisoned inoculum no degradation was noted after 14 days of exposure (based on DOC-measurements).

The reference item sodium benzoate was biodegraded by 99% within 7 days of exposure, confirming the suitability of the activated sludge.

In the toxicity control containing the test item, the reference item sodium benzoate and inoculum, the initial DOC decreased by 102% within 14 days of exposure. Thus, according to the test guidelines the test item can be assumed to be not inhibitory to activated sludge because degradation was >35% within 14 days.