Registration Dossier
Registration Dossier
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EC number: 605-301-6 | CAS number: 162691-59-0
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 79/831/EEC, Annex V, Part C
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-[3-(1-ethoxyethoxy)-3-methyl-4-penten-1-ynyl]-2,4,6,6-tetramethyl-5,6,7,7a-tetrahydro-1,3-benzodioxol-5-ol
- EC Number:
- 605-301-6
- Cas Number:
- 162691-59-0
- Molecular formula:
- C21 H32 O5
- IUPAC Name:
- 5-[3-(1-ethoxyethoxy)-3-methyl-4-penten-1-ynyl]-2,4,6,6-tetramethyl-5,6,7,7a-tetrahydro-1,3-benzodioxol-5-ol
- Details on test material:
- - Name of test material (as cited in study report): Asta-C15-Acetal (C15-Acl)
- Lot/batch No.: 25656/133 S
- Analytical purity: about 95 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (100 mg/l) was prepared by stirring the test substance in the test medium for about 20 hours at 20 °C.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.20 - 3.20 mmol/l
- Test temperature:
- 18.9 - 21.8 °C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 8.4 - 9.3 mg/l
- Nominal and measured concentrations:
- Nominal test substance concentrations:
6.25, 12.5, 25, 50 and 100 mg/l
Analytically determined test concentrations:
exposure time 0-24 h
after 0 h: 5.8 mg/l (nominal 6.25 mg/l), 24.2 mg/l (nominal 25 mg/l), 101.3 mg/l (nominal 100 mg/l)
after 24 h (stocked with animals): 5.6 mg/l (nominal 6.25 mg/l), 23.3 mg/l (nominal 25 mg/l), 95.9 mg/l (nominal 100 mg/l)
Exposure time 24-48 h
after 0 h: 5.8 mg/l (nominal 6.25 mg/l), 23.9 mg/l (nominal 25 mg/l), 96.0 mg/l (nominal 100 mg/l)
after 24 h (stocked with animals): 5.4 mg/l (nominal 6.25 mg/l), 23.5 mg/l (nominal 25 mg/l), 95.7 mg/l (nominal 100 mg/l) - Details on test conditions:
- TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- Renewal rate of test solution (frequency/flow rate): after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic M4 medium
- Alkalinity: 0.8 - 1.0 mmol/l
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 5 - 6 µE/(m²s) in the range of 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The test substance is not stable in the test medium.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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