Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 479-330-6 | CAS number: 67226-45-3
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
48-hour EC50 of 10 mg/L
Key value for chemical safety assessment
Additional information
A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 202, "Daphnia sp, Acute Immobilisation Test and Reproduction Test" referenced as Method C.2 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC), US Code of Federal Regulations, Title 40, Part 797, Section 1300 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.1010.
The test material was known to degrade immediately on contact with water forming N-butylpyridinium chloride, hydrochloric acid and aluminium oxides. Daphnids were exposed to an aqueous solution of the test material degradation products at concentrations of 1,0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L for 48 hours under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours. The 48-Hour EC50 for Daphnia magna based on nominal test concentrations was 10 mg/L with 95% confidence limits of 8.0 - 13 mg/L. The No Observed Effect Concentration was 3.2 mg/L. Chemical analysis of the test preparations was carried out using N-butylpyridinium chloride as a marker. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations of N-butylpyridinium chloride to be near nominal and so the results are based on nominal test concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
