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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 April 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
473-390-7
EC Name:
-
Cas Number:
1093615-61-2
Molecular formula:
C7F15NO
IUPAC Name:
2,2,3,3,5,5,6,6-octafluoro-4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)morpholine; 2,2,3,3,5,5,6,6-octafluoro-4-(1,1,2,2,3,3,3-heptafluoropropyl)morpholine
Constituent 2
Reference substance name:
MTDID 7145
IUPAC Name:
MTDID 7145
Details on test material:
- Name of test material (as cited in study report): MTDID 7145
- Substance type: Multiconstituent substance
- Physical state: Clear, colourless liquid
- Analytical purity: 94.5%
- Lot/batch no.: 142072:43
- Expiration date of the lot/batch: 30 Dec 2007
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Because of low solubility, FC-770 was added to reverse osmosis-purified water at a loading rate of 2000 mg/L, with 24 hours' stirring in closed 1-L test bottles. Note that during weighing some of the test substance was lost due to evaporation. Therefore, the initially weighed amount of test substance was slightly higher, i.e. approximately 520 mg. For the test, synthetic sewage feed (16 mL) and prepared, activated sludge (200 mL) were added to bring the loading rate to 1000 mg/L FC-770.

-Control: blanks, 3,5-Dichlorophenol as reference substance

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Laboratory culture: Sludge was collected from municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands.The sludge was coarsely sieved, washed and diluted with ISO medium. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.9 g/L of sludge, as used for the test). The sludge was collected one day before use. Before use the pH was checked (measured value: 6.1).

ISO medium (in reverse osmosis [RO]-purified tap water)
211.5 mg/L CaCl2∙2(H2O)
88.7 mg/L MgSO4∙7(H2O)
46.7 mg/L NaHCO3
4.2 mg/L KCl

ISO medium was used only for sludge preparation, not during the test.

- Method of cultivation: Because the sludge was used one day after collection, 50 mL of synthetic sewage feed (16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCI, 0.4 g CaCI2∙2(H2O), 0.2 g MgSO4∙7(H20), 2.8 g K2HPO4 dissolved in 1 L RO water and filtered. The pH was 7.2) was added to each litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Hardness:
Not reported
Test temperature:
18.2 - 20.4 °C
pH:
6.9 - 7.5
Dissolved oxygen:
Continuous aeration throughout exposure period. Measured dissolved oxygen [DO] values at start of observation period were 7.4-9.1 mg/L
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentration 1000 mg/L during exposure period. No analytical monitoring
Details on test conditions:
TEST SYSTEM
- Test vessel: Exposure (3 hours) in aerated 1-L bottles. Observation/DO measurement in sealed, 300 mL oxygen bottles.
- Type (delete if not applicable): Open during exposure. Closed during observation.
- Material, size, headspace, fill volume: 250 mL test solution, 16 mL synthetic sewage, and 200 mL sludge suspension plus sufficient RO water to bring total volume to 500 mL. Well mixed sample of the contents was poured into a 300 mL oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel (zero headspace).
- Aeration: Continuous aeration with pipette, and stirring
- No. of vessels per concentration (replicates): Two for FC-770, one for reference substance 3,5-dichlorophenol
- No. of vessels per control (replicates):Two
- Biomass loading rate: 1.56 g/L Mixed Liquor Sludge Solids

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges
- Culture medium different from test medium: Approximately 50 mL/L synthetic sewage added per day to sludge suspension during holding (maximum 4 days).
- Intervals of water quality measurement: Not specified. Water quality data not provided

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Light intensity: not specified

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen concentration

TEST CONCENTRATIONS
- Spacing factor for test concentrations: Single concentration tested
- Justification for using less concentrations than requested by guideline: Limit test, FC-770 not soluble in water.
Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: No reduction in respiration rate was recorded at nominal 1000 mg/L FC-770.
Details on results:
- Effect concentrations exceeding solubility of substance in test medium: yes
- Acceptability of controls: The difference between the control respiration rates was 13 % (criterion, <15 % variability).
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: The EC50 of 3,5-dichlorophenol was 5.4 mg/L (in the accepted range of 5-30 mg/L)

Any other information on results incl. tables

Table, Oxygen concentration, oxygen consumption, percentage inhibition and pH for Controls (C), 3,5-dichlorophenol (R), and FC-770 (T)

Replicate

Concentration of substance (mg/L)

Initial oxygen conc. (mg/L)

Oxygen consumption rate (mg O2/L/h)

Respiration inhibition (%)

pH

C1

0

7.6

39

6.9

C2

0

6.8

42

7.0

Mean C1+C2

40.5

R1

1.0

7.4

36

11

7.1

R2

3.2

7.9

26

36

7.5

R3

10

8.7

9

78

7.3

R4

32

9.1

3

93

7.2

C2

0

6.8

42

7.0

C3

0

7.9

37

7.0

Mean C2+C3

39.5

T1

1000

7.0

42

-6.3

6.9

T2

1000

7.8

41

-3.8

6.9

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Control respiration rate variability < 15%. Reference substance 3-h EC50 5.4 mg/L (within 5-30 mg/L)
Conclusions:
FC-770 is not inhibitory to activated sludge respiration (3-h EC50 >1000 mg/L)
Executive summary:

The toxicity of FC-770 to activated sludge organisms was assessed in an assay performed according to OECD TG209. The test solution was made by stirring FC-770 24 hours in a closed vessel at a loading rate of 2000 mg/L, with subsequence dilution to 1000 mg/L for the test. Undissolved FC-770 remained in the test bottles. The exposure period was for three hours with continuous aeration. No inhibition was observed upon exposure to FC-770 at the loading rate. The 3-hour EC50 to sludge is therefore >1000 mg/L. No NOEC is assigned due to volatility of FC-770.

The study was conducted in accord with GLP criteria and satisfies the guideline requirements for test method OECD209, activated sludge respiration inhibition test. It is classified as acceptable.

Results Synopsis

Test Type: Static                          

EC50:  >1000 mg/L                       95% C.I.:  not applicable

NOEC:  not applicable                        Probit Slope:  not applicable

Endpoint(s) Effected:  Respiration inhibition