A mixture of: (9-cis,12-cis)-2-hydroxy-3-(octadeca-9,12-dienoylamino)propyl hexadecanoate; (9-cis)-2-hydroxy-3-(octadeca-9-enoylamino)propylhexadecanoate ethyl hexadecanoate;

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 2014

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 301:1992

Deviations:

no

Principles of method if other than guideline:

The analysis of inorganic carbon for the evaluation in an aqueous biodegradability has been performed following screening method described in OECD 301:1992. Determination of total inorganic carbon has been done usig an automatic TOC analyser. It is a high sensitivity instrument based on the combustion catalytic oxidation method (680°C). The carbon dioxyde generated by oxidation has been detected using in infrared gas analyzer. Samples, properly diluted with deionised water with low TOC contents, have been analysed by means of an integrated sampling system directly from vials containing 40 ml volume.

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

other: whitish scales

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

A sample of activated sludge has been selected by the mixed treatment plant of urban (about 66%) and industrial (about 34%) liquid sewage

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

47.32 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

inorg. C analysis

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

118

Sampling time:

28 d

Remarks on result:

other: biodegradable

Details on results:

According to the OECD guidelines, a substance is considered biodegradable when its level of biodegradability is at least 60% within a period of 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

On the base of results obtained, interpreted in accordance to OECD 301 method, the test item Omega 6 Ceramide should be considered biodegradable in aerobic condition.

Executive summary:

On the base of results obtained, interpreted in accordance to OECD  301 method, the test item Omega 6 Ceramide should be considered biodegradable in aerobic condition.