Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
Method C1, Directive 92/69/EEC
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
430-910-7
EC Name:
-
Cas Number:
13335-71-2
Molecular formula:
Hill formula: C10H12O3 CAS formula: C10H12O3
IUPAC Name:
2-(2,6-dimethylphenoxy)acetic acid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples withdrawn at 0 and 24h

Test solutions

Vehicle:
no
Details on test solutions:
No auxiliary solvents were required.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Zebra fish: Brachydanio rerio
weight = 0.36g
length = 3cm

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
96 hours

Test conditions

Hardness:
247 mg of CaCO3
Test temperature:
23 oC
pH:
7.8
Dissolved oxygen:
8.4 mg/l
Nominal and measured concentrations:
Nominal conc= 10, 17, 28, 48, 80 mg/l
Measured conc = 9.56, 16.76, 28.05, 48.64, 81.61 mg/l
Details on test conditions:
exposed to artificial light 16 hours daily

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 45.91 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL lower = 37.72; 95% Cl upper = 55.87
Details on results:
First abnormalities observed at 48 mg/l at 72 hours
Reported statistics and error estimates:
LC 50: 95% CL lower = 37.72 mg/l ; 95% Cl upper = 55.87 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 for the test substance is estimated to be 45.91 mg/l at 96 hours