Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Data is for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

WoE report is based on three short term toxicity study of invertebrate for the test chemical :

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates
- Molecular formula :C8H22NO12P.Mo.W
- Molecular weight : 635.02 g/mol
- Smiles notation : CN(C)c1ccccc1_O=[Mo](=O)(O{-})O{-}_O=[W](=O)(O{-})O{-}_OP(O)(O)=O
- Substance type: Organic
- Physical state : Solid

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

3. Details on test organisms
TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD):< 24 h (hour(s))
Life Stage: JV (juvenile(s))

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

3. 8 degree C

Details on test conditions:

3. - No. of organisms per vessel: 5 daphnia magna

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.28 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Toxic from 1 study

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.27 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: For 2 study chemical was consider as toxic

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.072 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: For 3rd study

Validity criteria fulfilled:

not specified

Conclusions:

1. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate.
2. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l.
3. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.
Based on above experimental studies it was concluded that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Executive summary:

Summarized result for data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of aquatic invertebrates. The studies are as mentioned below:

 

In study 1 short term toxicity test was carried out for test chemical according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) on Daphnia magna for 48 hr exposure period. Test conducted under the static system and provide proper atmosphere as mention in the guideline. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.    

 

First study was supported by second in which aim of this study was to evaluate the nature of chemical when comes in contact with organisms by providing the exposure period of 48 hrs. Test conducted under the static system. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l. Based on the immobility, chemical was concluded as toxic and classified as aquatic acute 1/ chronic 2 as per the CLP criteria.

 

Similarly in third study short term toxicity to aquatic invertebrates was performed in static salt water condition for 48 hrs. <24 hrs old daphnia were used as a test organism. Test conducted under the static system in which 5 daphnia added in each vessel. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.

 

Based on above experimental studies it was concluded that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Description of key information

1. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate.

2. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l.

3. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded  to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.

Based on above experimental studies it was concluded that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.28 mg/L

Additional information

Summarized result for data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates on the growth of aquatic invertebrates. The studies are as mentioned below:

 

In study 1 short term toxicity test was carried out for test chemical according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) on Daphnia magna for 48 hr exposure period. Test conducted under the static system and provide proper atmosphere as mention in the guideline. Based on the mobility of daphnia magna by the test chemical, the effective concentration EC50 to 50% of daphnia magna for test chemical was determine to be 0.28 mg/l at 48 hr. From EC50, It can be concluded that the chemical was toxic to the aquatic invertebrate and thus can be considered to be classified in aquatic acute category 1 as per the classification criteria.    

 

First study was supported by second in which aim of this study was to evaluate the nature of chemical when comes in contact with organisms by providing the exposure period of 48 hrs. Test conducted under the static system. Study was conducted for 48 hrs and after that immobility was observed. Based on the immobility the EC50 was determine to be 0.27 mg/l. Based on the immobility, chemical was concluded as toxic and classified as aquatic acute 1/ chronic 2 as per the CLP criteria.

 

Similarly in third study short term toxicity to aquatic invertebrates was performed in static salt water condition for 48 hrs. <24 hrs old daphnia were used as a test organism. Test conducted under the static system in which 5 daphnia added in each vessel. After the exposure of 48 hrs immobility was observed in 50 % test organisms. The EC50 value for chemical was determined to be 0.072 mg/l. Based on the value the substance concluded to be toxic and can be considered to be classified in aquatic acute 1 as per the CLP regulations.

 

Based on above experimental studies it was concluded that the Benzenamine, N,N-dimethyl-, oxidized, molybdatetungstatephosphates was toxic and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.