9,10-Anthracenedione, 1,4-diamino-, N,N'-bis(4-C7-17-branched alkylphenyl) derivs.

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species, other

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Estimated data generated using the EPI Suite program developed by the USEPA.

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Prediction done using BCFBAF (v3.01) model

GLP compliance:

not specified

Radiolabelling:

not specified

Vehicle:

not specified

Test organisms (species):

other: Fish

Route of exposure:

aqueous

Test type:

not specified

Water / sediment media type:

natural water: freshwater

Test temperature:

25 deg.C

Reference substance (positive control):

not specified

Key result

Temp.:

25 °C

Type:

BCF

Value:

3.162 L/kg

Basis:

whole body w.w.

Calculation basis:

other: estimated

Remarks on result:

other: Non-bioaccumulative

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

Executive summary:

BCFBAF model (v3.01) of Estimation Programs Interface (EPI Suite, 2018) was used to predict the bioconcentration factor (BCF) of test chemical . The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

Description of key information

BCFBAF model (v3.01) of Estimation Programs Interface (EPI Suite, 2018) was used to predict the bioconcentration factor (BCF) of test chemical . The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

Key value for chemical safety assessment

BCF (aquatic species):

3.162 L/kg ww

Additional information

Predicted data for the target chemical and various supporting weight of evidence study for its structurally similar read across substance were reviewed for the bioaccumulation end point and their results are summarized as below:

 

In a prediction done using the BCFBAF Program(v3.01) of Estimation Programs Interface (EPI Suite, 2018) was used to predict the bioconcentration factor (BCF) of test chemical The bioconcentration factor (BCF) of test chemical was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the chemical is not expected to bioaccumulate in the food chain.

 

In a supporting weight of evidence study from authoritative database (HSDB & J-CHECK, 2017 and EnviChem, 2014) for the test chemical bioaccumulation experiment was conducted on test organism Cyprinus carpiofor 8 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 2.0mg/land 0.2 mg/l, respectively. Test chemical solution was prepared in HC0 20orHC0 20, respectively. Range finding study involve the TLm (48 hr) 1900 mg/l on Rice fish (Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 6.8 -20.4 L/Kg at a conc. of 2.0 mg/l and 13.2 -41.4 L/Kg at a conc. of 0.2 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

 

Another bioaccumulation study was conducted in fish for estimating the BCF (bioaccumulation factor) value of the test chemical (HSDB, 2017). The bioaccumulation factor (BCF) value was calculated using an estimated log Kow of 7.71 and a regression-derived equation. The estimated BCF (bioaccumulation factor) value of test chemical was determined to be 1520 dimensionless.

 

In Last study the bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemicaal (J-CHECK, 2017).The study was performed according to“OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)” andother guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1mg/land 0.1 mg/l, respectively. Test chemical solution was prepared in Crystal sugar or HCO-40.Analytical method involve the recovery ratio: Test water :Peak 1 87.1 %, Peak 2 97.4 %, Fish : Peak 1 86.5 %, Peak 2 83.8 %, - Limit of quantitation : Test water : 1st concentration area : Peak 1 0.043 mg/L, Peak 2 0.041 mg/L, 2nd concentration area : Peak 1 0.0043 mg/L, Peak 2 0.0041 mg/L, Fish : Peak 1 1.9 microg/g, Peak 2 2.1 microg/g. Range finding study involve the LC50 (48 hr) ≥ 130 mg/l (w/v) on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be4.3% at the start of exposure. The bioconcentration factor (BCF value) of test chemical onCyprinus carpio was determined to be in the range of 2.4 - 12 L/Kg at a conc. of 1 mg/l and ≤ 23 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

 

On the basis of above results for test chemical, it can be concluded that the BCF value of test substance was estimated to be3.162 L/kg, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.