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EC number: 305-673-7 | CAS number: 94944-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 July 2017 to 10 August 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Totnes Sewage Treatment Works, Totnes, Devon, UK
- Storage conditions: aerated at room temperature at pH 7.0 ± 1.0.
- Storage length: 2 days
- Preparation of inoculum for exposure: Six days prior to the exposure start the activated sludge was centrifuged, washed and re suspended in the mineral medium and the solids concentration determined. This sludge was then diluted in medium, added to test bottles and stirred until required for use. The seeded mineral medium was pre-conditioned for six days to reduce the blank oxygen uptake readings in the test.
- Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- calculated as BOD/COD
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 22 ± 1 °C
- pH: 7.4 ± 0.2 (actual 6.98-7.34)
- pH adjusted: no
- Sludge concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: dark glass 500 mL bottle placed in incubator
- Number of culture flasks/concentration: 3 (One replicate for the substance was excluded as an outlier (Dixon’s Q test)performed on day 10 results), this did not adversely affect the outcome of the study as two replicates are reported which complies with the minimum given in the OECD 301F guideline
- Method used to create aerobic conditions: continuously stirred
- Measuring equipment: Oxitop respirometer
- Details of trap for CO2 and volatile organics if used: KOH
Examiniations
- Frequency: daily
- Method: measurement of decrease in pressure over 240 min period
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (3 replicates)
- Abiotic sterile control: none
- Toxicity control: yes (3 replicates)
STATISTICAL METHODS: NA - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- other: % degradation BOD/COD
- Value:
- 5
- Sampling time:
- 28 d
- Details on results:
- Initial pH 7.36, at 28 days 7.41 for substance
Initial pH 7.2, at 28 days 7.3 for inoculum blank - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable
- Executive summary:
Subject
Determination of Ready Biodegradability
Guideline
OECD 301F: Manometric respirometry
Test concentration
Control and 100 mg/L
Length of test
28 days
Nominal
test temperature
22 ± 1°C
Mean degradation after 28 days
5%
Classification
Not readily biodegradable (Ref 1)
Reference substance
Sodium benzoate
Experimental values
substance
Reference substance
Day
Mean percentage biodegradation
Day
Mean percentage biodegradation
5
1
5
61
10
1
10
69
15
2
15
72
20
4
20
73
28
5
28
75
Comments
The COD of the test substance was calculated as2.04 g O2/g and the mean BOD value after 28 days was 0.10 g O2/g. The percentage degradation was calculated as (BOD/COD)´100
Conclusions
the substance attained a mean level of biodegradation (based on the BOD:COD ratio) of 5% after 28 days and the results showed good replication. Less than 60% degradation was achieved within 28 days, so the substance cannot be classified as readily biodegradable (Ref 1).
Reference
Day |
Biodegradation(%) Reference Substance |
Biodegradation(%) Substance |
Biodegradation(%) Tox control |
Mean |
Mean (based on 2 replicates) |
Mean |
|
1 |
1 |
1 |
1 |
2 |
42 |
1 |
19 |
3 |
52 |
0 |
23 |
4 |
58 |
0 |
28 |
5 |
61 |
1 |
30 |
6 |
64 |
1 |
32 |
7 |
66 |
0 |
32 |
8 |
68 |
0 |
34 |
9 |
68 |
0 |
35 |
10 |
69 |
1 |
35 |
11 |
70 |
1 |
36 |
12 |
72 |
0 |
36 |
13 |
71 |
2 |
37 |
14 |
71 |
2 |
37 |
15 |
72 |
2 |
38 |
16 |
72 |
3 |
37 |
17 |
73 |
3 |
38 |
18 |
72 |
2 |
38 |
19 |
73 |
2 |
39 |
20 |
73 |
4 |
39 |
21 |
73 |
3 |
40 |
22 |
73 |
4 |
40 |
23 |
73 |
4 |
40 |
24 |
75 |
5 |
40 |
25 |
75 |
5 |
41 |
26 |
75 |
4 |
41 |
27 |
74 |
4 |
41 |
28 |
75 |
5 |
42 |
Biodegradation (%) = Biological Oxygen Demand (g O2/g)× 100 / ThOD (1.67 g O2/g) and/or COD (1.94 g O2/g)
Description of key information
In a test according to OECD 301F the substance showed a biodegradation of 5% over the 28 day test period. Therefore the substance is considered not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.