Tri(tridecyl) benzene-1,2,4-tricarboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

EC Number:

302-446-4

EC Name:

Tri(tridecyl) benzene-1,2,4-tricarboxylate

Cas Number:

94109-09-8

Molecular formula:

C48H84O6

IUPAC Name:

tri(tridecyl) benzene-1,2,4-tricarboxylate

Test material form:

liquid

Details on test material:

- Moisture content: <= 0.1%

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Source : Lipo Chemicals Inc; Lot No: P-2960F-10
- Expiration date of the lot/batch: June 29, 2013
- Purity test date: > 99% (email dated February 17, 2011)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: : Not data available
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in house culture; original culture was obtained from Aquatic BioSystems (Fort Collins, CO) on 17 August 2006 (identified by Vittor and Associates, Mobile, AL as D. magna; 14 July 2003)
- Feeding during test : none

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:

Study design

Test type:

static

Water media type:

other: reconstituted water

Total exposure duration:

48 h

Test conditions

Hardness:

Hardness of 92 mg/L as CaCO3

Test temperature:

19.9 to 21.9°C

pH:

7.8

Dissolved oxygen:

8.7 to 9.0 mg/L

Salinity:

Na/K ratio 25.2

Conductivity:

312 microS/cm

Nominal and measured concentrations:

100 mg test item/L (Limit test)

Details on test conditions:

TEST SYSTEM
- Test vessel:250 mL glass beakers covered with Plexiglass
- Material, size, headspace, fill volume: Test volume 200 mL
- Aeration: before use in the test
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test item on < 24h old Daphnia magna neonates by observing the immobilisation of test organisms, over a 48h exposure period, under controlled conditions to 0 and 100 mg/L (WAF nominal concentrations) of the test item.
Results : 0% immobilisation observed in all of the replicates of the control and 100 mg test item/L nominal concentration.
EC50 (nominal) > 100 mg/L (48h)
LOEC (nominal) > 100 mg/L (48h)
NOEC (nomial) = 100 mg/L (48h)
Not harmful to Daphnia Magna