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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March-April 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
EC Number:
300-634-0
EC Name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
Cas Number:
93951-12-3
Molecular formula:
C28H39Cl2NO3
IUPAC Name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
Test material form:
solid
Details on test material:
Batch 44029
colour : White to off-white
retest date : 01/06/2019
Specific details on test material used for the study:
Controlled room temperature (15-25°C, ≤70% relative humidity), protected from light and humidity (store in a tightly closed container)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
Origin: The secondary effluent was supplied from the sewage plant for domestic sewage in Veszprém, Hungary.
Conditioning: The secondary effluent used for this study was allowed to settle approximately for an hour, then the decanted effluent was aerated until use (not later than an hour).
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
2.6 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
The test was carried out in an incubator and controlled environment room (during the formulation and oxygen measuring) at a temperature of 22 ± 2 °C according to guideline. The test flasks were placed into an incubator and kept at 20.6 - 23.2°C, in the dark. The temperature was measured on weekdays during the experiment.
The oxygen concentration of test water was 9.0 mg/L at the start of the test.
The pH value of the test water was checked prior start of the experiment. The pH of the test water was 7.30.
The test conditions were measured with suitable instruments and documented in the raw data.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Key result
Parameter:
% degradation (O2 consumption)
Value:
4.1
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
2.5
Sampling time:
21 d

BOD5 / COD results

Results with reference substance:
The reference item Sodium benzoate was sufficiently degraded to a mean of 80.8 % after 14 days, and to a mean of 92.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The test item was not considered to have significant inhibitory effects on the secondary effluent microorganisms.
Based on the results of this study, the test item is considered not readily biodegradable.
Executive summary:

The test item "80ACQ" was investigated for its ready biodegradability in a Closed Bottle Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

The test system was a microbial inoculum of good quality, collected from a sewage plant for domestic sewage. The sludge was allowed to settle and then aerated until use.

Under the test conditions the percentage biodegradation of 80ACQ reached a mean of 4.1 % after 28 days based on the ThOD of the test item. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThOD. Therefore, the test item is considered not readily biodegradable.

The reference item Sodium benzoate was sufficiently degraded to a mean of 80.8 % after 14 days, and to a mean of 92.5 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used inoculum.

In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 38.4 % biodegradation was noted within 14 days and 43.2% biodegradation after 28 days of incubation.

According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 2.6 mg/L on the secondary effluent microorganisms because degradation was >25 % within 14 days.

The test item was not considered to have significant inhibitory effects on the secondary effluent microorganisms.

Based on the results of this study, the test item is considered not readily biodegradable.