N-[4-(aminocarbonyl)phenyl]-4-methoxy-3-nitrobenzamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-[4-(aminocarbonyl)phenyl]-4-methoxy-3-nitrobenzamide

EC Number:

298-790-7

EC Name:

N-[4-(aminocarbonyl)phenyl]-4-methoxy-3-nitrobenzamide

Cas Number:

93839-20-4

Molecular formula:

C15H13N3O5

IUPAC Name:

N-(4-carbamoylphenyl)-4-methoxy-3-nitrobenzamide

Details on test material:

purity: 94.5% contains residual water

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The substance was suspended with 0.095 ml Tween 80/l to support the solution properties.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 bad Lauterberg
- Age at study initiation: about 3 months
- Length at study initiation: 3.1 +- 0.21 cm
- Feeding during test: twice a day, ad libitum
- Food type: Tetra Standard Mix, Tetra Werke, Melle

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Test temperature:

21.4 - 22.0 °C

pH:

7.3 - 8.2

Dissolved oxygen:

4.8 - 8.8 mg O2/L

Nominal and measured concentrations:

Nominal concentration: 500 mg/L
Analytically found after 0 hours: 272 mg/L; after 48 hours 98.8 mg/L; after 96 hours: 101 mg/L
average was calculated for 99.9 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 liter glass vessel, (30 cm x 22 cm x 24 cm), open
- Aeration: after 72 hours

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

99.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 99.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No change in appearance and behaviour

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the test result, the substance has not to be classifed for toxicity to the fish.

Executive summary:

The substance was tested for acute toxicity to the zebrabärbling according to OECD guideline 203. The nominal concentration was 500 mg/L. Due to the poor solubility of the substance the effective concentration was determined by HPLC and was found to be in average 99.9 mg/L.Tween 80/l was used to support the solubility. Tests were performed with 10 fish with 500 mg/L nominal and 0 mg/L , 0 mg/L plus Tween 80 as controls. There was no mortality and also no change in the appearance and the behaviour of the fish after 96 hours.

Therefore the LC50 is > 500 mg/L (nominal) rsp. > 99.9 mg/L (measured)