Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 295-835-2 | CAS number: 92129-33-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2.1 mg/L
Additional information
In the risk assessment report it is reported that the toxicity of DHTDMAC to Pseudomonas putida was investigated in a growth inhibition test according to a German DIN-guideline (Bringmann & Kühn method; UBA (1992). Experimentelle Überprüfung der Aussagekraft von Ergebnissen aus unterschiedlichen Bakterientoxiztätstests sowie vergleichende Untersuchungen zur Übertragbarkeit von Labortests auf reale Abwasser- und Gewässerbedingungen, unpublished report No. UBA-FB 106-03-069). In two tests EC50-values of 48 and 58 mg/L were derived after 18 hours (nominal values, graphically extrapolated).
Secondary effluent of a domestic wastewater treatment plant was used as inoculum in a closed bottle inhibition test (OECD 301D, UBA, 1992). The graphically extrapolated EC50-values of two tests were 2.0 and 6.5 mg/L (nominal concentrations) after a test duration of 5 days.
In an activated sludge respiration inhibition test (OECD 209) inoculum from a predominantly domestic wastewater treatment plant was used (UBA, 1992). A 3h EC50 of 520 mg/L was derived graphically from the dose response curve. The corresponding statistically derived value was 267
mg/L (nominal concentrations).
The toxicity of DHTDMAC to nitrifying bacteria enriched in a laboratory wastewater treatment plant (domestic sludge originally) was investigated in a manometric respirometer test (Wagner & Kayser, 1990). The test duration in the reference was referred to between 119 and 254 hours for different substances and was stopped when the nitrification of the controls was completed. The IC50 for inhibition of respiration was 2.1 mg/L active ingredient of DHTDMAC (a carrier solvent was used).
Anaerobic bacteria from a domestic wastewater treatment plant were exposed to DHTDMAC in an OECD 209 test (Hoechst AG (1989d). Prüfung des biologischen Abbaus von Genamin DSAC im OECD-Confirmatory-Test, unpublished report no. 434/89). The inhibition of respiration was measured after 3 hours and the EC10 was 80 mg/L, the EC50 = 220 mg/L.
Using different safety factors according to the sensitivities of the test systems and the mean effect values the lowest PNEC-values are as follows:
Pseudomonas putida EC50 = 53 mg/L, SF = 100 PNEC = 0.53 mg/L
Nitrifying bacteria EC50= 2.1 mg/L, SF = 10 PNEC = 0.21 mg/L
Secondary effluent EC50= 4.3 mg/L, SF = 100 PNEC = 0.043 mg/L
With all these PNECs it has to be considered that the microorganism toxicity derived in laboratory water tests has to be handled with care as a high influence of the composition of the wastewater (e.g. suspended particles, complexing agents) can be assumed, which is the same phenomenon as in surface water tests. Moreover the lowest PNECmicroorganisms of 0.043 mg/L seems to be unrealistic as it is reported that wastewater treatment plants operate at DHTDMAC concentrations of 3 to 8 mg/L. However, it is not documented whether the treatment process would be more effective without this DHTDMAC load in the influent and how less adapted plants might react. Nitrifying bacteria were found to be the most sensitive microorganisms with the lowest EC50 of 2.1 mg/L on which the risk assessment should be based (PNECmicroorganisms = 0.21 mg/L) to ensure that the most sensitive treatment process can take place.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.