Glycine, N-coco acyl derivs., sodium salts

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

10.01.2012 to 07.02.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Dr. U. Noack-Laboratorien, Käthe-Paulus-Straße 1, D-31157 Sarstedt, Germany

Test material

Test material information

Constituent 1

Reference substance name:

Glycine, N-coco acyl derivs., sodium salts

EC Number:

291-350-5

EC Name:

Glycine, N-coco acyl derivs., sodium salts

Cas Number:

90387-74-9

Molecular formula:

Molecular formula for this UVCB is not available.

IUPAC Name:

Glycine, N-coco acyl derivs., sodium salts

Test material form:

solid - liquid: aqueous solution

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No. of test material: Clariant Produkte (Deutschland) GmbH, Industriepark Höchst, Product Safety FUN, Geb. C 671, D-65926 Frankfurt am Main, Germany; Batch No.: ESD0003028
- Expiration date of the lot/batch: 21.10.2009
- Purity test date: not reported

STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, protected from light, in original container

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no

FORM AS APPLIED IN THE TEST (if different from that of starting material): Hostapon SG is a colourless aqueous liquid and was tested as such

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant of D-31137 Hildesheim (Germany)
- Preparation of inoculum for exposure: The activated sludge was washed twice with autoclaved tap water and concentrated to a dry sludge concentration of 3.0 g/L +/- 10%
- Pretreatment: no
- Initial biomass concentration: 3.05 g dry solids/L sludge; dry solid concentration in test solution: 1.53 g/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

20.5°C

pH:

7.5 +/-0.5

Dissolved oxygen:

>=60% of air saturation

Nominal and measured concentrations:

100, 180, 320, 580, and 1000 mg a.i./L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass/1000 mL/-/500 mL
- Aeration: aeration of test vessels by shaking
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): no vehicle used
- Sludge concentration (weight of dry solids per volume): 3.05 g dry solids/L sludge

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient solution: synthetic waste water according to OECD Guideline 209

OTHER TEST CONDITIONS
- Adjustment of pH: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): respiration inhibition

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using fewer concentrations than requested by guideline: -
- Range finding study: performed
- Test concentrations: 10, 100, and 1000 mg a.i./L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Copper (II) sulphate pentahydrate p.a.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

372 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Effect concentrations 3

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

791 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

The EC50 for respiration inhibition with the reference item copper (II) sulphate pentahydrate yielded a value of 129 mg/L. This value was within the validity range of 53-155 mg/L and thus the validity criterion for this aspect is fulfilled..

Reported statistics and error estimates:

The NOEC was determined determined via one way analysis of variance and Dunett's test. The EC-values of the Hostapon SG were calculated by sigmoidal dose-respnse regression. The EC50-value of the reference item was calculated by linear regression using the software GraphPad Prism5, GraphPad Software, Inc..

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC-, EC10, and EC50-values for the respiration inhibition of Hostapon SG were determined to be 320, 372, and 791 mg a.i./L nominal.

Executive summary:

The toxicity of Hostapon SG to activated sludge was investigated in a study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test). Activated sludge from a municipal sewage treatment plant treating predominantly domestic waste was exposed to Hostapon SG at different concentrations (range: 100 to 1000 mg a.i./L nominal). After 3 h of incubation the inhibition of respiration was determined in comparison to a set of test solutions without any test substance. The NOEC value for inhibition of total respiration was determined to be 320 mg a.i./L nominal, respectively. The corresponding EC10 and EC50 values were 372 and 791 mg a.i./L nominal, respectively.

The study is regarded as reliable without restriction and satisfies the requirements of the guideline.

Results Synopsis

Test organism: activated sludge from a municipal sewage treatment plant

NOEC: 320 mg a.i./L

EC10: 372 mg a.i./L

EC50: 791 mg a.i./L (nominal each)

Endpoint(s) Effected: respiration