1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No full study report, only summarised results

Principles of method if other than guideline:

No data concerning used method.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The test sample (1 mg/L) was introduced in acetone solution with a nominal acetone concentration of 500 ppm (v/v) in the test.

Test organisms (species):

Daphnia magna

Limit test:

yes

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

As would be expected for water insoluble lipophilic materials tested above their solubility limit most of the daphnia became trapped in a surface film ("floaters") and this place a stress on them. As time 24 hour the floating daphnia were re-submerged. After 24 and 48 h 0% Daphnia were immobilised.

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 after 48 h to Daphnia magna was greater than 1 mg/L under the conditions described.

Executive summary:

The LC50 after 48 h of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters was greater than 1 mg/L, tested with Daphnia magna.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-04-13 to 1994-04-15

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples of the freshly prepared stock solution and the dilution for testing after 0 and 48 hours were taken, no further details mentioned

Vehicle:

no

Details on test solutions:

As the test substance is poorly soluble in water, a 1 g/L mixture with synthetic freshwater was prepared. This mixture was stirred for 18 hours and filtrated afterwards. The filtrate was analyzed and showed an initial content of 7.4 mg/L DOC. The filtrate was used as Stock solution.
The analytical determination of stock and test solutions showed that they were not stable within 48 hours (deviation > 20%), therefore the geometric mean of the measured values after 0 and 48 hours were used for evaluation.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, clone 5
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 L beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of approx. 4 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.
- Feeding during test: none



ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

2.5 mmol

Test temperature:

20 °C ± 1 °C

pH:

7.9 - 8.2

Dissolved oxygen:

8.0 - 8.2 mg/L

Salinity:

CaCl2 x 2 H2O: 294 mg/L; MgSO4 x 7 H2O: 123 mg/L; NaHCO3: 63 mg/L; KCl: 5.5 mg/L
Ca/Mg ratio: 4:1
Na/K ratio: 10:1

Nominal and measured concentrations:

Nominal: 6.6 mg/L
Measured: 3.2 mg/L (geometric mean of measured concentrations after 0 and 48 hours)

Details on test conditions:

TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water
- Ca/mg ratio: 4:1
- Conductivity: not mentioned
- Culture medium different from test medium: yes
- Intervals of water quality measurement: not mentioned


OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours; O2 concentration, pH at the end of the test


TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Range finding study: not applicable, tested at solubility limit

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

dissolved

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

dissolved

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

>= 4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

dissolved

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

dissolved

Basis for effect:

mobility

Details on results:

The given concentrations were calculated from the estimated concentration of DOC. An estimation was neccessary, as the DOC value was decreasing in the progress of the test.

Results with reference substance (positive control):

- Results with reference substance valid? yes, historical control (not tested in parallel to the test substance)
- Concentrations: 1 and 2 mg/L potassium dichromate
- Results: 15% and 100% immobilised daphnia after 24 h

Reported statistics and error estimates:

Due to the low mortality up to the solubility limit, no statistics were performed.

Table #1: Effect of 1,2,4 -Benzenetricarboxylic acid, decyl octyl ester on immobilisation of Daphnia magna

concentration [mg/L]	24 hour values			48 hour values
	number		%	number		%
	mobile	immobile	immobile	mobile	immobile	immobile
0 (control)	19	1	5	19	1	5
3.2	20	0	0	19	1	5

The given concentration refers to the estimated content of DOC.

Remark: In the DOC-determination it was not possible to distinguish between carbon from the test substance and carbon from the impurities (2,5%)

Validity criteria fulfilled:

yes

Conclusions:

Not toxic under test conditions in the range of water solubility.

Executive summary:

The effect of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters on the immobilisation of Daphnia magna had been studied in accordance with OECD Guideline for Testing of Chemicals No. 202 part I and EEC Methods for Determination of Ecotoxicity, Annex to Directive 92/69/EEC.

Daphnia magna was exposed to 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters for an exposure time of 48 hours. As the test substance is poorly soluble in water, a 1 g/L mixture with synthetic freshwater was prepared. This mixture was stirred for 18 hours and filtrated afterwards. The filtrate was analyzed by means of DOC analysis and showed an initial content of 7.4 mg/L DOC. The filtrate was used as stock solution. The analytical determination of stock and test solution showed that they were not stable within 48 hours (deviation > 20%), therefore the geometric mean of the measured values after 0 and 48 hours for the test solution (3.2 mg/L) were used for evaluation.

No lethality or immobilisation of Daphnia magna > 5% was observed at the tested concentration after 48 hours exposure.

Description of key information

The test substance was not toxic under test conditions in the range of water solubility.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

> 1 mg/L

Additional information

The test substance was not toxic under test conditions in the range of water solubility.