Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with 2-propanamine

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

Oct. 4, 1989-Oct. 18, 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

Not applicable for UVCB

IUPAC Name:

sodium 4-undecylbenzenesulfonate

Sampling and analysis

Details on sampling:

Samples were from the 250 mg/kg dw concentration taken on day 0, 7, and 14. Measured concentrations were 235, 211, and 40.5 mg/kg dw respectively.

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

Test material was mixed with 1.16 L of deionized water, and then added to 2.44 kg of artificial soil.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Vittor and Associates, Mobile Alabama
- Age at test initiation (mean and range, SD): adult
- Weight at test initiation (mean and range, SD): average 0.66 g

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Total exposure duration:

14 d

Test conditions

Test temperature:

19-22 degrees C

pH:

5.5-5.8

Moisture:

34-36%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): jars with perforated lids
- Amount of soil or substrate: 900 g moist soil
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil)
- % sand: 70
- % clay: 20, also 10% sphagnum peat
- Soil classification system: sandy loam


OTHER TEST CONDITIONS
- Photoperiod: constant lighting
- Light intensity: ~600 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, health, behaviour, and number of cocoons on days 7 and 14

Nominal and measured concentrations:

Nominal: 0, 63, 125, 250, 500, and 1000 mg/kg dw

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: body weight

Effect concentrations 2

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

EC10

Effect conc.:

277 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: body weight

Remarks on result:

other: EC10 was not reported in original study report, but later calculated using study results using the method of Vanewijk, PH, and Hoekstra, JA, 1993. Calculation of the EC50 and its confidence interval when subtoxic stimulus is present. Ecotoxicol. Environ

Details on results:

There was 5% mortality at the 1000 mg/kg dw concentration on day 14. Body weight was decreased in the 500 and 1000 mg/kg dw groups by 23 and 33% respectively. The 1000 mg/kg dw group also showed lethargy and burrowing was also affected. Cocoon production was diminished in the 250 mg/kg dw group, and inhibited at higher concentrations.

Any other information on results incl. tables

Mortality and Weight Loss of Earthworms

Concentration	Number of			% Weight Loss
(mg/kg dw)	Day 0	Day 7	Day 14	Day 0-14
Control	40	40	40	14
63	40	40	40	5
125	40	40	40	6
250	40	40	40	5
500	40	40	40	23
1000	40	38	37	33

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 14-day LC50 to earthworms was > 1000 mg/kg dw soil. The 14-day NOEC was 250 mg/kg dw soil.

Executive summary:

Groups of earthworms were exposed to concentrations of 0, 63, 125, 250, 500, and 1000 mg/kg dw for 14 days. The test animals were examined for mortality, behaviour, health, and reproduction on days 7 and 14. 5% mortality was seen at the highest concentration. Effects to health were seen at the 500 and 1000 mg/kg dw concentrations. The 14 -day LC50 was > 1000 mg/kg dw, and the NOEC was 250 mg/kg dw.