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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed in accordance with OECD Guideline and compliant with GLP criteria.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
EC Number:
284-183-4
EC Name:
(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
Cas Number:
84803-57-6
Molecular formula:
C14H19N
IUPAC Name:
2-(4-tert-butyl-2,6-dimethylphenyl)acetonitrile
Details on test material:
- Test substance name: 2,6-Dimethyl-4-tert.-butylbenzylcyanid
- Aggregate state: Solid powder
- Colour: White
- Homogeneity: Homogeneous
- Storage conditions: Ambient

Sampling and analysis

Analytical monitoring:
not required

Test solutions

Details on test solutions:
The test substance was added in the required amount to the test vessel with about 180 mL deionized water. The mixture was stirred for 22 ± 2 hours to obtain an optimal solution. Then synthetic medium was added, the pH-value was adjusted to 7.5 ± 0.5, the inoculum was added and the test was started.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge f rom laboratory wastewater plant treating municipal sewage. The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 0.5 °C
pH:
6.8-8
Dissolved oxygen:
- Before adding inoculum: 6.8 - 7.2 mg/L (pH corrected)
- After 3 hours inubation: 8.0
Nominal and measured concentrations:
- Nominal concentration: 0 (control) and 1000 mg/L
Details on test conditions:
- Test vessels: Erlenmeyer-vessel (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
- Oxygen concentration during aeration: >2.5 mg/L
- Oxygen concentration immediately before measurement: >6.5 mg/L
- Duration of the measurement of oxygen consumption: 8 - 10 min
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
No change in oxygen consumption rate was observed at 1000 mg/L
Results with reference substance (positive control):
3-h EC20: ca. 3 mg/L
3-h EC50: ca. 22 mg/L

Applicant's summary and conclusion