Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Principles of method if other than guideline:
None
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Trisodium 2-[[6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)methylamino]-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate
EC Number:
274-700-1
EC Name:
Trisodium 2-[[6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)methylamino]-1-hydroxy-3-sulphonato-2-naphthyl]azo]naphthalene-1,5-disulphonate
Cas Number:
70616-89-6
Molecular formula:
C24H18ClN7O10S3.3Na
IUPAC Name:
trisodium 2-({6-[(4-amino-6-chloro-1,3,5-triazin-2-yl)(methyl)amino]-1-hydroxy-3-sulfonato-2-naphthyl}diazenyl)naphthalene-1,5-disulfonate
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
None

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Activated sludge of a communal sewage treatment plant Ara Reinach collected on 01.11.94
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
150 mg/L
Based on:
DOC
Details on study design:
Experimental conditionsWater / Nutrient: according to the specifications of the OECD guidelineTemperature: 20 - 25 °C (room temperature)Test duration: 28 daysLighting: indirect daylightEstimation Technique: TOC/DOC Analyzer 500
Reference substance
Reference substance:
diethylene glycol

Results and discussion

Preliminary study:
None
Test performance:
None
% Degradation
Key result
Parameter:
% degradation (DOC removal)
Value:
4
Sampling time:
28 d
Remarks on result:
other: Standard deviation not available
Details on results:
None

BOD5 / COD results

Results with reference substance:
The biodegradation of the reference substance after 8 days was: 98 %

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Biodegradation of the test substance after 28 days was 4 %.
Executive summary:

A key study was performed to determine the percent biodegradation of FAT 40170. An inherent biodegradability of FAT 40170 was determined in a modified Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance was tested in a concentration of 150 mg/l. The reference substance (Diethylene glycol) was tested in a concentration of 150 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 4 %.