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EC number: 279-976-7 | CAS number: 82486-82-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- This study was conducted between 31 August 2017 and 28 September 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-(butylnitroamino)ethyl nitrate
- EC Number:
- 279-976-7
- EC Name:
- 2-(butylnitroamino)ethyl nitrate
- Cas Number:
- 82486-82-6
- Molecular formula:
- C6H13N3O5
- IUPAC Name:
- butyl(nitro)[2-(nitrooxy)ethyl]amine
Constituent 1
- Specific details on test material used for the study:
- Name : Butyl-NENA
NIVA GLP substance number: G128
CAS : 82486-82-6
Batch number : 02/16, alternative 160002
Appearance : Yellow liquid, oily
Purity : 100 %. Added stabilizer n-Methyl p-Nitro Aniline, 0.5 %
Solubility in water : 100-500 ppm.
ThODNH4 : 0.70 mgO2/mg ThODNO3: 1.62 mgO2/mg
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: marine
- Details on inoculum:
- The seawater was pumped from a depth of 60 meter in the Oslofjord, from NIVA's Research Station at Solbergstrand, and collected in 30 L polyethylene containers. The temperature is logged at the research station and not measured according to GLP.
Appearance: Clear
Salinity: 35‰
Temperature: 8.1 ˚C
Dissolved organic carbon concentration was analyzed after storage (1.1 mg C/L). The concentration of heterotrophic bacteria was also determined (1.3x105 CFU/mL) by plating on marine agar and incubated for 8 days at 25 ± 2 °C. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- DOC removal
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- other: Nitrification
- Details on study design:
- Preparation of seawater test medium
The seawater was transported to NIVA in Oslo and stored at test temperature for three days in the dark. Water (15 L) was siphoned into new can and aerated for 1.5 hours. Nutrient stock solutions A, B, C and D (OECD 306) were added (1 ml/L). The composition of the stock solutions is shown in Table 1.
Preparation of test bottles
Blank control
Test bottles (15 pc) were filled by siphoning from the seawater test medium container and sealed with a glass stopper.
Test sample bottles
Test substance was added to the test bottle by using an inert support technique (ISO 10634): approximately 0.9 mg butyl-NENA was added to glass cover slip, weight measured exactly on balance, and added to the empty test bottles. The bottles (15pc) where filled carefully with seawater, allowing minimum overflow when adding the glass stopper. The weight of the test bottles was measured before and after addition of test water to calculate the exact volume in each bottle.
Reference compound bottles (test control)
A stock solution of aniline (C6H5NH2, 99 %, Mw 93.13) was prepared as the reference (test control) by dissolving 0.2722 g aniline in 100 ml MilliQ water. A container was filled with 5 L seawater test media by siphoning and added 5 ml aniline stock solution and mixed. Test bottles (12 pc) were filled by siphoning from this container and sealed with a glass stopper.
Toxicity control
Bottles for toxicity control where added test substance as described for test bottles, and filled with the same stock of seawater media added aniline as described for the reference bottles.
Incubation and sampling
All bottles (blank, test, reference and toxicity) were incubated under dark plastic in a temperature controlled room (21.2 ± 1.6oC).
The dissolved oxygen (DO) concentration was measured on days 0, 7, 14, 21 and 28.
Both Butyl-NENA and aniline are nitrogen containing compounds and the ThOD depends on whether the nitrogen is degraded to ammonium or oxidized further to nitrite and nitrate. The concentrations of nitrite and nitrate were measured in all bottles after DO-measurements.
Physical and chemical measurements
Chemical analysis
• DOC: Tekmar Dohrmann Apollo 9000 HS using NIVA method G5-3 (based on modified NS-EN 1484:1997)
• Nitrate and nitrite: Skalar San Plus Autoanalysator using NIVA method D 3-3 (based on modified NS-4745).
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 11
- Sampling time:
- 28 d
- Details on results:
- Temperature
Temperature range during test was maximum 22.7”C and minimum 19.6”C, resulting in a mean test temperature of 21.2 ± 1.6”C
BOD5 / COD results
BOD5 / COD
- Key result
- Parameter:
- ThOD
- Value:
- 11 other: %
- Results with reference substance:
- Aniline was used as the reference compound in the test to assess the viability of the indigenous bacteria used to evaluate the biodegradability of the test compound. The biodegradability of this reference compound should be at least 60% of the reference material Aniline (i.e. >60% of its theoretical oxygen demand; ThOD) within 28 days and at least 50% within a timeframe of 2-12 days ending latest at day 19 after commencing the test as according to a EEC ring-test (Nyholm and Kristensen).
The biodegradation of aniline in this experiment was 63% after 7 day’s incubation and 70% after 14 days (ThODNH4). No nitrification occurred. The criteria of 50% degradation within 12 days and >60% within 28 days are fulfilled.
Any other information on results incl. tables
Nitrification assessment
Because both Butyl-NENA and Aniline are nitrogen containing substances, the possibility of nitrification must be assessed by comparing the concentration of nitrite + nitrate during the incubation period.
No increase in nitrite + nitrate was observed in the blank or the reference controls. The test bottles with Butyl-NENA and the toxicity control had a higher starting concentration of nitrate and nitrite than the blank, however, there is no significant increase of the average nitrite + nitrate concentration in the test bottles compared to the starting concentration even if some of the bottles had higher concentrations.
Validity of test
Blank control
The dissolved oxygen in the blank control bottles decreased 6% (from 7.20 mg/L to 6.74 mg/L) over the 28 days’ test period. This is within the acceptable limit of 30%.
Toxicity control
The oxygen consumption in the toxicity control was comparable to the sum of the oxygen consumption from the reference control and the test substance separately, confirming no inhibition of the bacteria from Butyl-NENA.
Biodegradation of the test samples
The test substance Butyl-NENA had very little biodegradation in this test, with 11% reduction of ThOD after 28 days incubation assuming no nitrification.
Table1: Nitrification in test, reference control and toxicity control
|
NO2+NO3 concentration [µg N/L] |
||||||||
Sample |
Initial conc. |
d7 |
Average ± SD |
d14 |
Average ± SD |
d21 |
Average ± SD |
d28 |
Average ± SD |
Blank |
131 |
126 |
131 ± 5 |
116 |
118 ± 4 |
121 |
121 ± 2 |
109 |
111 |
135 |
122 |
120 |
6 |
||||||
132 |
115 |
123 |
113 |
||||||
G128 |
149 |
195 |
197 ± 8 |
175 |
180 ± 13 |
170 |
173 ± 3 |
175 |
157 ± 27 |
205 |
195 |
175 |
170 |
||||||
190 |
170 |
175 |
126 |
||||||
Ref |
127 |
130 |
130 |
118 |
124 |
108 |
116 |
|
|
130 |
130 |
123 |
|||||||
Tox |
180 |
195 |
200 |
175 |
175 |
170 |
173 |
|
|
205 |
175 |
175 |
|
Table 2 ThOD and Oxygen Consumption
ThODNH4 |
||||||
G128 |
0,70 |
|||||
day |
0 |
7 |
14 |
21 |
28 |
|
Oxygen consumption |
0 |
0,17 |
0,22 |
0,22 |
0,22 |
|
% of ThOD NH4 |
0 |
8 % |
10 % |
10 % |
11 % |
|
ThODNH4 |
||||||
Reference |
2,41 |
concentration |
2,722 |
mg/L |
||
day |
0 |
7 |
14 |
21 |
||
Oxygen consumption |
0 |
4,13 |
4,62 |
4,62 |
||
% of ThOD NH4 |
0 |
63 % |
70 % |
71 % |
||
Tox |
|
|||||
day |
0 |
7 |
14 |
21 |
||
Oxygen consumption |
0 |
4,23 |
4,64 |
4,95 |
||
% of ThOD NH4 |
0 |
49 % |
52 % |
57 % |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of the study indicate that Butyl-NENA (3 mg/L) was 11% biodegraded in seawater over an incubation period of 28 days.
- Executive summary:
The biodegradability of Butyl-NENA has been assessed in seawater at 21.2 ± 1.6˚C using the Closed Bottle Test method according to OECD Guidelines for Testing of Chemicals 306 (OECD, 1992) modified for addition of poorly soluble substances.
The test compound was exposed to indigenous microorganisms in natural seawater.Butyl-NENAwas tested at a concentration of 3.1 ± 0.1 mg/L, corresponding to a ThOD of 5.0 mgO2/L.
The biodegradation of Butyl-NENA in this test was 11% after 28 days, assuming full nitrification. No inhibitory effect of the microorganisms was assessed during the study.
The reference control, aniline, reached 63% degradation within seven days and thus confirmed the viability of the microorganisms in the seawater used.
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