Pentasodium bis[5-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-4-hydroxy-3-[(2-hydroxy-5-nitrophenyl)azo]naphthalene-2,7-disulphonato(4-)]chromate(5-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Details on test conditions (pH, hardness, etc.) missing in report.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

other: internal method B-21 equivalent to OECD209

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

activated sludge

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

3,5-Dichlorophenol showed an inhibition of 90.8% when tested at 100 mg/l and 83.7% at a concentration of 32 mg/l. At 10 mg/l, the inhibition was 15.3%, resulting in an IC50 (3,5-Dichlorophenol) of 18 mg/l.

Test substance concentration [mg/l]	Oxygen consumption [mg/l and min]	Inhibition [%]
400	0.97	1.0
128	0.97	1.0
40	0.89	9.2

Validity criteria fulfilled:

yes

Conclusions:

The IC50 (3h) was determined to be >400 mg/l.

Executive summary:

In this inhibition test on sewage sludge bacteria the IC50 (3h) was determined to be >400 mg/l and thus the substance FAT 40210 is considered to be of low acute toxicity towards sewage sludge bacteria. A classification according to CLP (Regulation (EC) No 1272/2008) is not required.

Description of key information

A key study was performed to determine the toxicity of the test substance in microorganisms. In this inhibition test on sewage sludge bacteria the IC50(3h) was determined to be >400 mg/l and thus the test substance is considered to be of low acute toxicity towards sewage sludge bacteria. A key study was performed to determine the toxicity of the test substance in microorganisms. In this inhibition test on sewage sludge bacteria the IC50(3h) was determined to be >400 mg/l and thus the test substance is considered to be of low acute toxicity towards sewage sludge bacteria. A classification according to CLP (Regulation (EC) No 1272/2008) is not required.

Key value for chemical safety assessment

EC50 for microorganisms:

400 mg/L