Sodium 2(or 5)-[[1-amino-4-[[3-[[(chloroacetyl)amino]methyl]-2,4,6-trimethylphenyl]amino]-9,10-dihydro-9,10-dioxo-2-anthryl]oxy]-5(or 2)-(tert-butyl)benzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

July 1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: According to in-house SOP (A.11) devloped by CIBA comparable to today's standard guidlines

Deviations:

not specified

Principles of method if other than guideline:

Internal SOP followed.

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

other: fish (Trout)

Details on test organisms:

None

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

13 °C

pH:

7.9 to 8.0

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

Nominal concentrations: 2, 20 and 100 mg/l

Details on test conditions:

No data

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was seen at 2 mg/L, while 1 fish was found dead at 20 mg/L after 48 h exposure. At 100 mg/L, no exposed fish survived.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

None

Validity criteria fulfilled:

not applicable

Conclusions:

The LC50 of the test substance was found to be 40 mg/L.

Executive summary:

The acute fish toxicity of FAT 21016/A was assessed in a study conducted accoding to a methodology similar to OECD 203. Groups of Rainbow trouts, each containing 8 fish, were exposed to the test concentrations of 2, 20 and 100 mg/L for 48 h. No mortality was seen at 2 mg/L, while 1 fish was found dead at 20 mg/L after 48 h exposure. At 100 mg/L, no exposed fish survived. Hence, the LC50 of the test substance was found to be 40 mg/L.

Description of key information

The acute toxicity of Acid Violet 109 was assesed in couple studies conducted using methodology similar to OECD Guideline 203. In the study considered as key, groups of Rainbow trouts, each containing 8 fish, were exposed to the test concentrations of 2, 20 and 100 mg/L for 48 h. No mortality was seen at 2 mg/L, while 1 fish was found dead at 20 mg/L after 48 h exposure. At 100 mg/L, no exposed fish survived. Hence, the LC50 of the test substance was found to be 40 mg/L. However, in a supporting study, no mortality was observed when groups of fish were exposed to FAT 20200/A at the test concentrations of 100, 200 and 1000 mg/L for 48 h. Hence, the TL₅₀ of Acid Violet 109 in this study was found to be 1000 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

40 mg/L