Benzyl(3-hydroxyphenacyl)methylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl(3-hydroxyphenacyl)methylammonium chloride

EC Number:

276-017-4

EC Name:

Benzyl(3-hydroxyphenacyl)methylammonium chloride

Cas Number:

71786-67-9

Molecular formula:

C16H17NO2.ClH

IUPAC Name:

benzyl(3-hydroxyphenacyl)methylammonium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Benzyladrianon HCl
- Physical state: solid
- Analytical purity: 99.07 %
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L

Test temperature:

18 - 22 C°

pH:

8.8 - 8.2

Nominal and measured concentrations:

At the start the measured concentrations were in agremment or just below nominal concentrations ( 87 % - 94 % ).
During the 24-hour period between renewal the concentrations measured remain within 80 % of the initially measured concentration ( 78 % - 84 % of
nominal ).
The concentrations measured in the freshly prepared solution at 24 hours of exposure showed that the procedure of preparation was repeatable
( 98 % -95 % of nominal ).

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL , all-glass , filling 80 mL
- Aeration: No aeration of test vesssel
- No. of organisms per vessel: 10
- No. of vessels per concentration : 2

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

45 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0

Results with reference substance (positive control):

- Results with reference substance valid? The acutal response in this reference test with potassium dichromate are within the ranges of the expected responses at the different concentrations. Hence, the sensitivity of this batch of D.magna was in agreement with the historical data collected at NOTOX
- 24 h EC50 > 1.8 mg/L
- 48 h EC50 = 0.95 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study Benzyladrianon HCl did not induce acute immobilisation of Daphnia magna at 10 mg/L after 48 hours of
exposure.
The 24h-EC50 was 45 mg/L based on nominal exposure concentrations ( 95 % confidence interval between 42 and 50 mg/L).
The 48h-EC50 was 19 mg/L based on nominal exposure concentrations ( 95 % confidence interval between 17 and 22 mg/L) .

Executive summary:

Acute Toxicity Studey with Daphnia magna with Benzyladrianon HCl.

After a static range-finding test, a final EC50 test was performed with Daphnia magna exposed for a maximum of 48 hours to range of nominal concentrations from 10 to 100 mg/L. The test was performed under semi-static conditions with renewal of the test solution after 24 hours of exposure. The test was performed in duplicate with 10 daphnia per vessel. Samples were taken from the freshly prepared solutions at the start and the 24 hour old and from the freshly prepared solutions after 24 hour exposure.

At the start the measured concentrations were in agreement or just below nominal concentrations ( 87 % - 94 % ).

During the 24-hour period between renewal the concentrations measured remain within 80 % of the initially measured concentration

( 78 % - 84 % of nominal ).The concentrations measured in the freshly prepared solution at 24 hours of exposure showed that the procedure of preparation was repeatable ( 98 % -95 % of nominal ). Hence, the procedure of preparation was repeatble and toxicity parameters were based on the nominal concentartions.

Under the conditions of the present study Benzyladrianon HCl did not induce acute immobilisation of Daphnia magna at 10 mg/L after 48 hours of exposure. The 24h-EC50 was 45 mg/L based on nominal exposure concentrations ( 95 % confidence interval between 42 and 50 mg/L). The 48h-EC50 was 19 mg/L based on nominal exposure concentrations ( 95 % confidence interval between 17 and 22 mg/L).