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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
EC Number:
275-177-2
EC Name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
Cas Number:
71077-34-4
Molecular formula:
C14H14N2O4S.Na
IUPAC Name:
sodium 4-[(3-amino-4-methylbenzoyl)amino]benzenesulfonate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida Migula, DSM 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
22°C
Reference substance (positive control):
yes
Remarks:
2,4-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
16 h
Dose descriptor:
other: inhibitionrate
Effect conc.:
1 328 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -24.9%
Duration:
16 h
Dose descriptor:
other: inhibitionrate
Effect conc.:
664 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -11.1%
Duration:
16 h
Dose descriptor:
other: inhibition rate
Effect conc.:
332 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: -9.9%
Details on results:
The substance supports an slight increase of the growth of the bacteria
Results with reference substance (positive control):
2,4-dichlorophenol
30 mg/L : 11.1 % growth inhibition
90 mg/L: 79.7% growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for bacteria is greater then 1328 mg/L
Executive summary:

A growth inhibition test according DIN 38 412 Teil 8 for Pseudomonas putida was performed with the substance using concentrations of 1328, 664 and 332 mg/L.

There was inhibition observed but a slight increase of the growth of the bacteria