Sodium 6-amino-5-[[2-[(ethylphenylamino)sulphonyl]phenyl]azo]-4-hydroxynaphthalene-2-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June from 01st to 11th, 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

GLP compliance:

not specified

Details on test solutions:

Direct dispersion in water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout.
- Source: Parkwood Trout Farm, Kent, U.K.
- Length at study initiation: 4.3 ± 0.3 cm
- Weight at study initiation: 0.64 ± 0.20 g

ACCLIMATION
- Health during acclimation: no deaths occurred during the 7 days prior to the start of the study.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 litre
- Aeration: yes.
- Renewal rate of test solution: daily.
- No. of vessels per concentration: 1 replicate per concentration in the range-finding test; two replicates in the main test.
- No. of vessels per control: one replicate in both range-finding and main tests.
- Biomass loading rate: 0.32 g bodyweght/litre.

TEST MEDIUM / WATER PARAMETERS
- Dilution water: dechlorinated tap water (sodium thiosulphate).
- Hardness: approximately 50 mg/l as CaCO3.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

MAIN TEST

Concentration (mg/l)	Cumulative mortality (initial population: 10)
	24 hrs	48 hrs	72 hrs	96 hrs
Control	0	0	0	0
100	0	0	0	0
100	0	0	0	0

RANGE-FINDING TEST

Concentration (mg/l)	Cumulative mortality (initial population: 3)
	24 hrs	48 hrs	72 hrs	96 hrs
Control	0	0	0	0
1	0	0	0	0
10	0	0	0	0
100	0	0	0	0

Conclusions:

LC50 (96h) > 100 mg/l (nominal)

Executive summary:

The acute toxicity potential on fish was investigated following the procedures outlined into the OECD guideline 203.

First, a range-finding experiment was performed at the concentrations of 1, 10 and 100 mg/l. No deaths occurred, thus 100 mg/l was chosed as concentration for the main study. Also in this case no death was recorded.

Conclusion

LC50 (96h) > 100 mg/l (nominal)

Description of key information

LC50 (96h) > 100 mg/l (nominal)

Additional information

The acute toxicity potential on fish was investigated following the procedures outlined into the OECD guideline 203.

First, a range-finding experiment was performed at the concentrations of 1, 10 and 100 mg/l. No deaths occurred, thus 100 mg/l was chosed as concentration for the main study. Also in this case no death was recorded.

Although concentrations during the test were not measured, the substance is expected to be stable over the experiment period. Acid Red 057 is water soluble; it is not readily biodegradable and, based on the chemical structure, it is not expected to undergo hydrolysis.