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EC number: 271-867-2 | CAS number: 68610-51-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 February - 22 March 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study performed according to DIN guideline and according to GLP principles. Due to the water insolubility of the test substance, the guideline method was modified. Although the DIN method is not the preferred method under REACH, the results can be used for the calculation of the PNEC for microorganisms when no other test results are available (ECHA Guidance on information requirements and chemical safety assessment, chapter R.7b, section R.7.8.17.1)
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- Due to the water insolubility of the test substance, the test was modified. For details see below.
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on test solutions:
- Test 1: aqueous test suspension:
Test substance and Tween 80 (0.1 g/L) were mixed in ultrapure water for 15 min in an ultrasonic bath. Then the suspension was mixed overnight at 50°C using a magnetic stirrer, after which the test solution was filtered and the filtrate was used in the test and diluted.
Test 2: ethanol-based test suspension:
A suspension of test substance and ethanol was prepared and mixed in ultrapure water for 15 min in an ultrasonic bath. Then the suspension was mixed overnight at 50°C using a magnetic stirrer, after which it was used in the test and diluted. The highest test concentration contained some undissolved test substance. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Stockculture: DSM-No. 50026, lyophilisate from 29 Jan 1991.
Stock cultures were inoculated weekly in stock agar. These cultures (max. 7 days old) were used in the test. To adapt the cultures to fluid media, three 250 mL Erlenmeyer flasks were inoculated with bacteria in 100 mL of nutritional preculture solution. After 18+/-2 hours at 21°C, opacity was determined and set at FNU 100 using preculture medium. This suspension was used as the inoculum for the test-preculture.
Then 90 mL of preculture medium was inoculated with 10 mL of bacterial suspension (FNU 100). This bacterial suspension (FNU 10) was incubated for 7h at 21°C and subsequently the opacity was set at 50 FNU. This suspension was used as the inoculum in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 17 h
- Post exposure observation period:
- not applicable
- Hardness:
- no data
- Test temperature:
- 21 +/- 1°C
- pH:
- Test 1: pH of undiluted filtrate was 5.79
Test 2: pH of highest test concentration solution was 6.52 - Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Test 1: 10000, 6250, 3125, 1562.5 and 781.3 mg/L
Test 2: 150.9, 94.3, 47.2, 23.6 and 11.8 mg/L. - Details on test conditions:
- For each test concentration, 3 test cultures and one test substance control (i.e. no inoculum) were used. In 250 mL erlenmeyer flasks, 100 mL of test solution + bacterial suspension (FNU 50; 10 ml) were added. Controls (i.e. without test substance but with Tween 80 or ethanol) were also tested.
All test cultures were incubated for 17h at 21°C at a rotation shaker. - Reference substance (positive control):
- no
- Duration:
- 17 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- filtrate of aqueous suspension
- Basis for effect:
- growth inhibition
- Duration:
- 17 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 150.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- solved in ethanol and diluted in water
- Basis for effect:
- growth inhibition
- Details on results:
- Test 1: Growth inhibition at 10000, 6250, 3125, 152.5 and 781.3 mg/L: 4.4%, -6.8%, -4.6%, -3.4% and -5.9%, respectively.
Test 2: Growth inhibition at 150.9, 54.3, 7.2, 23.6 and 11.8 mg/L: -30.8, -8.5, -6.9, -6.9 and -4.3%, respectively. - Validity criteria fulfilled:
- yes
- Remarks:
- The opacity of the control cultures at the start of the test (i.e. 5 FNU) increased at least 100 times during the test.
- Conclusions:
- The test substance does not induce growth inhibition of Pneudomonas putida when tested as filtrated test solutions up to a concentration of 10000 mg/L or when tested after being dissolved in ethanol and then diluted in water up to a concentration of 150.9 mg/L. The measured pH values of the highest test concentrations, pH 5.79 and pH 6.52, respectively, did not affect the cell multiplication.
Reference
Description of key information
One GLP study is presented.
The IBR study was conducted according to DIN 38412 (part 8) and exposed Pseudomonas putida for 17h in a static test to nominal concentrations up to 10000 mg/L of Lowinox CPL (filtrate of an aqueous suspension) and up to 150.9 mg/L (first dissolved in ethanol and then diluted in water). The EC50 was >150.9 mg/L and the NOEC was 150.9 mg/L, based on the lowest nominal concentrations.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 150.9 mg/L
- EC10 or NOEC for microorganisms:
- 150.9 mg/L
Additional information
Due to the low water solubility, the test substance solutions in the IBR study were prepared in two ways. The first stock solution was prepared by dissolving the test substance in water in the presence of Tween 80, ultrasonicating for 15 min and stirring at 50°C overnight. The filtrate of this suspension was used as the highest test concentration (10000 mg/L) and for further dilutions.
The second stock solution was prepared by dissolving the test substance in ethanol and subsequent mixing with water, ultrasonicating for 15 min and overnight stirring at 50°C. This suspension with visible undissolved material was used as the highest test concentration (150.9 mg/L) and for further dilutions.
The bacteria did not show any effects on growth. It can be concluded that the substance does not cause growth effects in bacteria at concentrations which are higher than its water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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