Hydrogen tetrakis[2,2-bis[(allyloxy)methyl]butan-1-olato-O1]bis(ditridecyl phosphito -O'')titanate(2-)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 - 23 Sep 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1992

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen tetrakis[2,2-bis[(allyloxy)methyl]butan-1-olato-O1]bis(ditridecyl phosphito -O'')titanate(2-)

EC Number:

264-709-9

EC Name:

Hydrogen tetrakis[2,2-bis[(allyloxy)methyl]butan-1-olato-O1]bis(ditridecyl phosphito -O'')titanate(2-)

Cas Number:

64157-14-8

Molecular formula:

not applicable (UVCB substance)

IUPAC Name:

dihydrogen 2-{[(di{[bis(tridecyloxy)phosphanyl]oxido}tris({2,2-bis[(prop-2-en-1-yloxy)methyl]butyl}oxido)titaniotetrakis(ylium))oxido]methyl}-1-(prop-2-en-1-yloxy)-2-[(prop-2-en-1-yloxy)methyl]butane

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): collected from the Easton Wastewater Treatment Facility, Easton, Maryland, USA on September 17, 2018. The Easton facility treats predominantly residential wastes
- Preparation of inoculum for exposure: The sludge was sieved using a 2-mm screen and adjusted to approximately 1000 mg total suspended solids/L with mineral media and then aerated at test temperature until use
- Concentration of sludge: 1000 mg total suspended solids/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

5.267 mg/L

Based on:

DOC

Remarks:

mean of three replicates

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium:
2470 mL of high grade water
3 mL calcium chloride solution (2.75%)
3 mL of ferric chloride solution (0.025%)
3 mL of magnesium sulfate solution (2.25%)
30 mL of phosphate buffer (pH 7.4)
- Test temperature: 19.15-21.60 ºC
- pH: 7.57-7.62
- Suspended solids concentration: ≤30 mg/L

TEST SYSTEM
- Culturing apparatus: amber 4-liter bottles
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: air entering the chambers was passed through Drierite™ to remove ambient moisture and then through Ascarite® to produce CO2-free air.
- Details of trap for CO2 and volatile organics if used: 0.5 M potassium hydroxide (KOH)
- Measuring equipment: Shimadzu Model TOC-VCSH carbon analyzer

SAMPLING
- Sampling frequency: days 3, 6, 8, 11, 15, 19, 22, 26 and 28


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 replicates
- Reference control: yes, 3 replicates
- Toxicity control: yes, 1 replicate (10 mg C/L reference and test substances respectively).

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

40.3

Sampling time:

28 d

Remarks on result:

other: average of 3 replicates

Details on results:

The toxicity control achieved >25% degradation by Day 14 and therefore the test substance is considered non-inhibitory at the concentration tested in this study.

BOD5 / COD results

Results with reference substance:

Biodegradation rate Sodium benzoate >60% based on CO2 on day 6

Any other information on results incl. tables

Table 1: Cumulative Percent of Theoretical Carbon Dioxide Evolved

Day	Blank control (mean of three replicates)	Reference substance replicate 1	Reference substance replicate 2	Reference substance replicate 3	Mean	Test substance replicate 1	Test substance replicate 2	Test substance replicate 3	mean	Toxicity control
3	NA	35.7	40.4	42.0	39.37	0.9	-4.3	1.5	-0.63	15.0
6	NA	64.0	71.2	67.4	67.53	2.1	1.6	3.2	2.30	29.4
8	NA	76.6	81.6	77.8	78.67	2.5	4.4	4.3	3.73	34.8
11	NA	84.9	89.4	85.4	86.57	4.7	7.6	4.3	5.53	41.8
15	NA	89.4	93.8	90.5	91.23	10.5	14.1	7.7	10.77	49.9
19	NA	91.7	95.7	92.8	93.40	20.6	23.6	13.5	19.23	55.1
22	NA	94.1	97.9	96.4	96.13	28.7	30.3	18.9	25.97	59.6
26	NA	94.4	98.8	99.3	97.50	35.9	36.1	27.3	33.10	63.3
29	NA	94.1	99.9	100.8	98.27	43.7	43.0	34.3	40.33	70.7
Cumulative Average Day 29 (n=3)		98.3				40.3				70.7
Standard dev.		3.6				5.2

Table 2: Validity criteria for OECD 301.

Criterion from the guideline	Outcome	Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.	<20%	yes
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%).	67.53 % at day 6	yes
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d.	49.9% at day 14	yes
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC.	n.d.	n.d.
The total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium.	18 mg CO2/L	yes

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.

Interpretation of results:

other: not readily biodegradable according to OECD criteria