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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
27.-29.07.1993
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate
EC Number:
258-946-7
EC Name:
2-[[(4-methoxyphenyl)methylhydrazono]methyl]-1,3,3-trimethyl-3H-indolium methyl sulphate
Cas Number:
54060-92-3
Molecular formula:
C20H24N3O.CH3O4S C21H27N3O5S
IUPAC Name:
2-{[2-(4-methoxyphenyl)-2-methylhydrazin-1-ylidene]methyl}-1,3,3-trimethyl-3H-indol-1-ium methyl sulfate
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dilution from stock solution

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
No data
Test temperature:
21.3 - 22 °C
pH:
8.0 - 8.1
Dissolved oxygen:
97 - 99 %
Nominal and measured concentrations:
Nominal: 0 (blank control), 0.01, 0.10, 1.0, 10.0 and 100 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.316 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: at 0.1 mg/L no immobile daphnia after 48h; at 1.0 mg/L 10 (of 10) Daphnia immobile after 48 h

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 of the test item to daphnia is 0.32 mg/L.
Executive summary:

The 48–hr-acute toxicity of the test item to Daphnia was studied at nominal concentrations of 0 (blank control), 0.01, 0.10, 1.0, 10, and 100 mg/L. The 48– hour EC50was determined to be 0.3162 mg/L.