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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
02/08/1994-04/08/1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Norme Francaise AFNOR T 90 301 de janvier 1983

Test material

Specific details on test material used for the study:
The test solutions were prepared in different ways according to their physicochemical properties:
- Soluble products (powders and liquids): preparation of a test solution at 1 g / l.
- for not soluble liquids: production of a stable emulsion, 1 g/l solution treated for 5 mins with ultrasound (20 kHz, 50 watts)
- for not soluble powders: preparation of a test solution of 1 g of product in 20 ml of DMSO

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20°C +/- 2°C
pH:
2.27

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.4 mg/L

Any other information on results incl. tables

 concentration

 N° of daphnia immobilized after 48 h

Total / 20

  % of immobilisation  pH  O2 mg/l
 0  0 0     
 solvent control 2% 2   10    
 0.65  2  10    
 0.9  4  20    
 1.3  8  40    
 1.8  14  70    
 2.5  16  80    
 3.5  20  100 7.93   6.6

EC(I)50 -48h calculated by the computerized method of calculation of PROIBITS (95% confidence interval): 1.40 mg/l (1.20 -1.63 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The percentage of daphnia immobilized within 48 hours in the control tubes is 0% and is therefore ≤ 10%.
The conditions are simultaneously satisfied. The results obtained for the 26 products studied can therefore be considered valid.