Bis(isopropyl)naphthalene

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Only secondary literature (tables), based on UBA test programme

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38412, part 8

Principles of method if other than guideline:

Growth inhibition test with the bacterium Pseudomonas putida / Test conducted by WaBoLu; no report available.

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

in stock solution only

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated stock solution (water-accommodated fraction) was prepared by mechanically stirring of excess DIPN per 1 L medium for 48 h followed by separation of the insoluble material from the water phase using pre-conditioned glass fiber filter. The stock solution was diluted with dilution water to the desired concentration.

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Test temperature:

25 °C

Nominal and measured concentrations:

no data

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Aeration: no

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412, part 8

OTHER TEST CONDITIONS
- Adjustment of pH: no


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth inhibition

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

> 0.16 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Validity criteria fulfilled:

not specified

Conclusions:

No growth inhibition within the range of water solubility.

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Abstract / only short communication, based on UBA test programme

Principles of method if other than guideline:

Method: Bioluminescence inhibition assay / test conducted by WaBoLu, BGA/Berlin; no report available.

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Vibrio fisheri

Details on inoculum:

no data

Test type:

static

Water media type:

saltwater

Total exposure duration:

15 min

Details on test conditions:

TEST SYSTEM

no data

Reference substance (positive control):

not specified

Duration:

15 min

Dose descriptor:

EC50

Effect conc.:

> 0.1 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

No inhibitory effect within the range of water solubility.

Reference 3

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

Method: Comparative study programme on the applicability and validation of this test system

GLP compliance:

not specified

Analytical monitoring:

no

Details on test solutions:

DIPN was solubilized and finely dispersed in DMSO/medium.

Test organisms (species):

Tetrahymena pyriformis

Details on inoculum:

no data

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Hardness:

no data

Nominal and measured concentrations:

no data

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 0.15 mg/L

No EC50 could be derived, no data available on effects induced
by DIPN. 

It can be concluded that DIPN produced no toxicity within its water solubility. 
Documentation in tabular form only.
   

Validity criteria fulfilled:

not specified

Conclusions:

No inhibitory effect within the range of solubility

Description of key information

Diisopropylnaphthalene does not exhibit inhibitory effects on the microorganism tested within its range of water solubility (0.1 to 0.2 mg/L).

Key value for chemical safety assessment

EC50 for microorganisms:

0.15 mg/L

Additional information

For assessment of the toxicity to microorganisms, three studies have been identified. Due to limited reporting (secondary literature or short communication in tabular form), the reliability is 4. As results are very similar, they are used in a weight of evidence approach.

 

Study	Test concentration	Result	Experimental conditions	Reliability
UBA/KBwS 1999 (Pseudomonas putida, growth inhibition)	no individual test concentrations reported	EC10: > 0.16 mg/L (no growth inhibition within the range of water solubility)	no vehicle 16 h static	4 (WoE) (secondary literature)
UBA/KBwS 1999 (Vibrio fischeri, luminescence inhibition)	no individual test concentrations reported	EC50: > 0.1 mg/L (no inhibitory effect within the range of water solubility)	no vehicle 15 min static saltwater	4 (WoE) (secondary literature)
Yoshioka 1985 (Tetrahymena pyriformis,	no individual test concentrations reported	EC0: > 0.15 mg/L (no toxicity within the range of water solubility)	Vehicle: DMSO 24 h static	4 (WoE) (short communication)

 

Based on data reported, it is concluded that diisopropylnaphthalene does not exhibit inhibitory effects on the organism tested within its range of water solubility (0.1 to 0.2 mg/L).