α,α,6,6-tetramethylbicyclo[3.1.1]hept-2-ene-2-propionaldehyde

Short-term toxicity to fish

Description of key information

A study was performed to assess the acute toxicity of the substance to fish (Cyprinus carpio). The study was conducted in accordance with OECD TG 203 and GLP. In a semi-static test set-up in air-tight closed vessels (fill volume: 6.2 L), seven fish per concentration were exposed to 5 concentrations of the substance in test water (10, 18, 32, 56 and 100% of a saturated solution prepared at a loading rate of 100 mg/L) and a control. Throughout the test, test solutions were refreshed in intervals of 24 hours. After 3.5 hours all fish in the two highest treatment groups had died. Hypoactive swimming was observed in the test group at 0.76 mg/L after 24 and 48 hours, whereas no abnormalities were observed after 3.5, 72 and 96 hours. In the test group at 1.3 mg/L, incidences of mortality were observed within 24 hours, as well as loss of equilibrium and/or immobility at all other timepoints. Based on mortality the following effect value was determined: 96 -h LC50 = 1.5 mg/L. All results are expressed in terms of mean measured concentrations. Initial measured concentrations in fresh solutions at test start and at t=72 hours were comparable and decreased in used solutions to 52 -78 % of initial during the first refreshment period of 24 hours, and to 49 -54% of initial during the last refreshment period of 24 hours. Therefore, geometric mean measured concentrations were calculated for the first and last time interval, and these values were used to calculate the 0 -96 h (arithmetic) mean measured concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.5 mg/L