2-phenyl-1H-benzimidazole-5-sulphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because a long-term aquatic toxicity study on invertebrates is available

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

from Oct. 05, 1989 to Oct. 23, 1989

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

Old OECD 202 guideline test with GLP, but the exposure period is only 24h, and the determination of the stability, homogenity, and concentration of the test material in the test medium was not reported.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

only 24h test duration

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

Preparation of test medium: stock solution (1100 mg/L)

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna Straus parthenogenetic females
Basic strain: clone-breed of the Federal Board of Health; Berlin
Test animals: laboratory-bred by Bayer AG Leverkusen
Age of the animals: 6 - 24 hours

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

no data

Test temperature:

19.3 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.7 mg/L

Salinity:

no data

Nominal and measured concentrations:

nominal: 707 mg/L, 1000 mg/L

Details on test conditions:

Number of duplicate determinations: 2
Test volume: 20 mL
Number of daphnias per test vessel: 10

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Both of the immobility values at the two test concentrations (707 mg/l and 1000 mg/l) after 24 hours exposure are 0 %.

Results with reference substance (positive control):

EC0: 2 mg/L
EC100: > 5 mg/L
The sensitivity of the employed laboratoty population of Daphnia magna to the reference substance potassium dichromate has shown striking drift phenomena in recent years. The EC50 is no longer within the concentration range of 0.9 mg/L - 1.9 mg/L stated in the above-mentioned test guideline.

Validity criteria fulfilled:

yes

Conclusions:

The EC0 was determined to be above 1000 mg/L (nominal conc.).

Executive summary:

The test was performed according to the old test guideline of OECD 202 under GLP compliance. Both of the immobility values at the two test concentrations (707 mg/L and 1000 mg/L) after 24 hours exposure are 0 %. This result showed that the EC0 was determined to be above 1000 mg/L (nominal conc.).

Description of key information

The short-term toxicity to aquatic invertebrates was tested in with an old OECD 202 test over 24h (EC50 > 1000 mg/L) [disregarded]. Additionaly a long-term toxicity study according to OECD 211 was conducted. Within this study the EC50(48h) was determined to be > 100 mg/L (highest tested concentration).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L

Additional information

The short-term toxicity to aquatic invertebrates is assessed with a long-term study with aquatic invertebrates. Within this long-term study the EC50(48h) was determined to be > 100 mg/L.

A dissregarded acute daphnia test is also available. It was performed according to the old test guideline of OECD 202 over 24h under GLP compliance. Both of the immobility values at the two test concentrations (707 mg/L and 1000 mg/L) after 24 hours exposure are 0 %. This result showed that the EC0 (24h) was determined to be above 1000 mg/L (nominal conc.).