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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Link to relevant study record(s)

Description of key information

OECD Guideline 301C, GLP, key study, validity 1:
-4% (by BOD) and -2% (by GC) biodegradation after 28 days;
Not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

To assess the biodegradation potential of the registered substance, one experimental key study is available.

This study (Kurume, 2005) was performed on the registered substance according to OECD Guideline No. 301C with GLP compliance. A measured volume of inoculated mineral medium (suspended sludge concentration at 30 mg/L), containing a known concentration of test substance (100 mg/L) is stirred in a closed flask at a constant temperature (25 ± 1°C) for up to 28 days. Percentage biodegradation by BOD and determination of test item by gas chromatography (GC) were measured. Degradation of the reference substance (aniline) exceeds 40 % after 7 days and 65 % after 14 days, therefore the activity of the inoculum is verified and the test is considered valid. The percentage of biodegradation of the test substance by BOD is -4% and by GC is -2%. In conclusion, the test substance was not biodegraded by microorganisms under the present test conditions. The registered substance is not readily biodegradable.