N,N'-hexane-1,6-diylbis[3-(3,5-di-tert-butyl-4-hydroxyphenylpropionamide]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-09-26 to 1988-10-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

, vehicle concentration 155 mg per liter test water, no analytical dose verification

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 10.0 g of the test substance were mixed with 398 mg alkylphenol-polyglykol-ether and made up to 25 mL with DMF
- Controls: Blank control with test medium only and vehicle control with 151 mg DMF and 4 mg alkylphenol-polyglykol-ether per liter water in the concentration used for the test concentration
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): alkylphenol-polyglykol-ether and DMF
- Stock solution: 10.0 g of the substance were mixed with 398 mg alkylphenol-polyglykol-ether and made up to 25 mL with DMF.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Yes. Small parts of the test substance were swimming at the surface of the test solution from the start of the test. A slight deposit was observed after 24h exposure.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: West Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 24 mm (19-27 mm)
- Weight at study initiation (mean and range, SD): 0.12 g (0.05-0.17 g)
- Loading: 0.08 g/L
- Feeding during test: None

ACCLIMATION
- Acclimation period: 19 d
- Adaption: 24 hours / no food 24 hours prior to exposure

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg CaCO3/L

Test temperature:

23 ± 1°C

pH:

8.1-8.4

Dissolved oxygen:

85-100%

Nominal and measured concentrations:

Nominal: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.08 g/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water (carbon filter)
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 hours daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, Symptoms

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation): Small parts of the test substance were swimming at the surface of the test solution from the start of the test. A slight deposit was observed after 24h exposure.
- Effect concentrations exceeding solubility of substance in test medium: Yes

Executive summary:

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to test item at nominal concentrations of 0 (control, solvent control) and 100 mg/L under static conditions.  The 96-h LC50 was > 100 mg/L.  The NOEC, based on mortality and sublethal effects, were ≥ 100 mg/L, respectively.  Sublethal effects were not observed.

Description of key information

With high probability acutely not harmful to fish. 

Additional information

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to test item at nominal concentrations of 0 (control, solvent control) and 100 mg/L under static conditions. The 96-h LC50 was > 100 mg/L. The NOEC, based on mortality and sublethal effects, were ≥ 100 mg/L, respectively. Sublethal effects were not observed. It can be concluded that the test substance is not acutely toxic to fish in the range of solubility.