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EC number: 243-654-4 | CAS number: 20262-76-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity study of microorganism for the test chemical :1.To evalaute toxicity of test material on Paramaecium caudatum2.Toxicity to micro-organisms was carried out for test material for 14.68 to study the effects on micro-organisms.
- Specific details on test material used for the study:
- Name of the test chemical: Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonateCommon Name: Patent Blue V Sodium saltMolecular Formula: C27H32N2O7S2.NaMolecular Weight: 582.6709 g/molSMILES Notation: CCN(CC)C1=CC=C(C=C1)C(=C2C=CC(=[N+](CC)CC)C=C2)C3=CC(=C(C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+]InChI: 1S/C27H32N2O7S2.Na/c1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;+1/p-1
- Test organisms (species):
- Paramaecium caudatum
- Details on inoculum:
- 2) Cultures of Paramecium caudatum (PC): PC was maintained at 22°C on 0.15 % dried lettuce infusion and fed with Aerobacter aerogenes.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 17.283 min
- Test temperature:
- 22°C
- Duration:
- 17.283 min
- Dose descriptor:
- other: LT50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: motality
- Duration:
- 14.68 min
- Dose descriptor:
- other: LT50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical is not likely to be toxic to microorganism atleast in the concentration of 1000 - 10000 mg/l
- Executive summary:
Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:
1.The Median Lethal Time for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 17.283 min. study on the basis of mortality effect was estimated to be 1000 mg/L. It can be concluded from the value that the test materialis not toxic to the aquatic enviornment.
2.Toxicity to micro-organisms was carried out for 14.68 to study the effects on micro-organisms. The Median Lethal Time value for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 14.68 min. study on the basis of mortality effect was found to be 10000 mg/L. It can be concluded from the value that the test material is not toxic to the aquatic enviornment.
On the basis of above LT50 concentrations test material Sodium 4-{[4-(diethylamino)phenyl] [4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}- 6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4) is considered to be non toxic to microorganisms.
Reference
Description of key information
Toxicity to microorganism:
Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:
1.The Median Lethal Time for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 17.283 min. study on the basis of mortality effect was estimated to be 1000 mg/L. It can be concluded from the value that the test materialis not toxic to the aquatic enviornment.
2.Toxicity to micro-organisms was carried out for 14.68 to study the effects on micro-organisms. The Median Lethal Time value for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 14.68 min. study on the basis of mortality effect was found to be 10000 mg/L. It can be concluded from the value that the test material is not toxic to the aquatic enviornment.
On the basis of above LT50 concentrations test material Sodium 4-{[4-(diethylamino)phenyl] [4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}- 6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4) is considered to be non toxic to microorganisms.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Toxicity to microorganism:
Data available for the structurally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies are as mentioned below:
1.The Median Lethal Time for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 17.283 min. study on the basis of mortality effect was estimated to be 1000 mg/L. It can be concluded from the value that the test materialis not toxic to the aquatic enviornment.
2.Toxicity to micro-organisms was carried out for 14.68 to study the effects on micro-organisms. The Median Lethal Time value for 50 percent mortality (LT50) value of test material in microorganism (Paramaecium caudatum) in a 14.68 min. study on the basis of mortality effect was found to be 10000 mg/L. It can be concluded from the value that the test material is not toxic to the aquatic enviornment.
On the basis of above LT50 concentrations test material Sodium 4-{[4-(diethylamino)phenyl] [4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}- 6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4) is considered to be non toxic to microorganisms.
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