Hydrogen [4-[4-(diethylamino)-5'-hydroxy-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, monosodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :1.Acute Toxicity Study of test material on Zebra fish (Danio rerio) in a Static System (96 Hours)2.Acute Toxicity Study of test material on Fish in a Static System

Specific details on test material used for the study:

Name of the test chemical: Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonateCommon Name: Patent Blue V Sodium saltMolecular Formula: C27H32N2O7S2.NaMolecular Weight: 582.6709 g/molSMILES Notation: CCN(CC)C1=CC=C(C=C1)C(=C2C=CC(=[N+](CC)CC)C=C2)C3=CC(=C(C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+]InChI: 1S/C27H32N2O7S2.Na/c1-5-28(6-2)21-13-9-19(10-14-21)27(20-11-15-22(16-12-20)29(7-3)8-4)23-17-24(30)26(38(34,35)36)18-25(23)37(31,32)33;/h9-18H,5-8H2,1-4H3,(H2-,30,31,32,33,34,35,36);/q;+1/p-1

Analytical monitoring:

not specified

Details on test solutions:

1.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L, respectively. 2.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L ,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

1.Animal species: Zebra Fish (Danio rerio)Animal breeder/ supplier: Asim Fancy Fish Aquarium, DharampethBody length: Average 2.46 cmBody weight: Average 0.094 g2.TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source:KOYAL AQUARIUM, Dharampeth, Nagpur, MS (India)- Average length: 1.79 cm- Body weight 0.1204g (average)- Physical conditions: No abnormalities- Food type:Standard brand feed,- Frequency: once daily- Duration of housing 15 days - Photoperiod 16 hours light and 8 hours dark-Aeration Continuous

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

1) 81.5 (mg of CaCO3)2) 148.5 (mg of CaCO3)

Test temperature:

1) 25 degree C

pH:

7.1

Dissolved oxygen:

2) 7.4 mg/L

Salinity:

1) 1175 TDS (ppm)513 Conductivity (µScm-1)

Nominal and measured concentrations:

1) 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L (Nominal) 2) 100 mg/l

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate is not likely to be toxic to fish atleast in the concentration of >100 mg/l

Executive summary:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies

are as mentioned below:

1.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L, respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentrations, 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

2.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L ,respectively

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentration 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies

are as mentioned below:

1.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L, respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentrations, 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

2.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L ,respectively

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentration 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical Sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)-2,5-cyclohexadien-1-ylidene]methyl}-6-hydroxy-1,3-benzenedisulfonate (20262 -76 -4).The studies

are as mentioned below:

1.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L, respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentrations, 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.

2.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L ,respectively

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item was subjected to various nominal test concentration 100mg/L . LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was non toxic and can be consider to be not classified as per the CLP classification criteria.