Bis(5-oxo-L-prolinato-N1,O2)copper

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical follow-up, no hardness data; but the results demonstrate the adequacy of the test system

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Bis(5-oxo-L-prolinato-N1,O2)copper

EC Number:

239-471-4

EC Name:

Bis(5-oxo-L-prolinato-N1,O2)copper

Cas Number:

15454-74-7

Molecular formula:

C10H12CuN2O6

IUPAC Name:

bis(5-oxo-L-prolinato-N1,O2)copper

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

algae culture medium

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

no data

Test temperature:

23°C +/- 2°C

pH:

7.80

Nominal and measured concentrations:

nominal: 0, 0.1, 0.17, 0.31, 0.56, 1 mg/L
analysed: not applicable

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.34 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 0.1 - < 0.34 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Remarks on result:

other: EC10 was not calculated in the report, but its range can be determined using the report's raw data: as only 1% inhibition of growth rate was observed at 0.10 mg/L nominal, it can be concluded that EC10 ranges 0.1 (EC1) to 0.34 (EC50) mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Algae 72h- EC50 (static assay, growth rate) is 0.340 mg/L.
EC10 was not calculated in the report, but its range can be determined using the report's raw data: as only 1% inhibition of growth rate was observed at 0.10 mg/L nominal, it can be concluded that EC10 ranges 0.1 (EC1) to 0.34 (EC50) mg/L.