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EC number: 236-670-8 | CAS number: 13463-40-6
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions. No analytical dose verification was performed.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1990
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 Part 9, 1982.
- GLP compliance:
- no
Test material
- Reference substance name:
- Pentacarbonyliron
- EC Number:
- 236-670-8
- EC Name:
- Pentacarbonyliron
- Cas Number:
- 13463-40-6
- Molecular formula:
- C5FeO5
- IUPAC Name:
- pentacarbonyliron
- Details on test material:
- Purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 244 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 119 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Results of test BASF AG, 1989, 89/1320
During the 72 h exposure to Pentacarbonyliron fluorescence values were as follows (average of 4 replicates)*:
Nominal test fluorometer values after x hours
concentration
(mg/l) 0 24 48 72
-----------------------------------------------------------
Control 51 95 293 925
Solvent contr. 55 121 448 1954
3.9 52 143 533 1759
7.8 52 141 520 1701
15.625 50 131 510 1670
31.25 53 112 318 1188
62.5 49 110 302 1084
125 50 109 323 1079
250 48 85 133 563
500 48 29 15 19
-----------------------------------------------------------
*10000 cells/ml were introduced at the test start.
ECx values (in mg/l) after x hours of exposure
24 h 48 h 72 h
---------------------------------------------------------------------
EC20 188.1 28 19.1
EC50 357.5 190.2 149.5
EC90 - 435.5 418.3
---------------------------------------------------------------------
No chemical dose verification was performed. All values refer to nominal test concentrations of Pentacarbonyliron.
EC values are related to solubilizer control.
Remark: Strong growth support by solubilzer Chremophor RH 40 was observed.
---------------------------------------------------------------------
In a new mathematical evaluation, critical concentrations of BASF AG Algae test 89/1320, has been re-calculated, in order to allow an assessment based on growth rate and biomass (according to the requirements given in OECD guideline 201). (BASF AG 2007, 2/1320/89)
The mathematical evaluation was based on a linear relationship between fluorescence and cell number and raw data were equated with cell numbers.
Summary of Results for all Endpoints: Critical effect and threshold concentration as observed at end of experimental time; EC: Effective concentration for xx% reduction; LOEC: Lowest observed effect concentration; NOEC: No observed effect concentration
a) Yield
Critical conc.s
(mg/L) 0-24h 0-48h 0-72h
EC10 143.18 25.11 7.44
EC20 190.99 44.65 16.83
EC50 330.43 133.45 79.57
LOEC 31.25 31.25 <3.90
NOEC 15.60 15.60 <3.90
b) Growth rate
EC10 218.42 85.27 81.78
EC20 237.91 117.87 119.14
EC50 279.91 218.25 243.73
LOEC 250.00 31.25 31.25
NOEC 125.00 15.60 15.60
c) Sectional growth rate
EC10 218.42 85.44 30.04
EC20 237.91 113.31 76.55
EC50 279.91 193.88 453.69
LOEC 250.00 31.25 <3.90
NOEC 125.00 15.60 <3.90
d) Biomass integral
EC10 98.35 31.11 14.93
EC20 132.77 52.46 28.50
EC50 235.00 141.80 97.51
LOEC 31.25 31.25 15.60
NOEC 15.60 15.60 7.80
Remark: for 95% Confidence limits, please see BASF AG (2007), 2/1320/89
Applicant's summary and conclusion
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