Pentacarbonyliron

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. No analytical dose verification was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

13436-40-6

IUPAC Name:

13436-40-6

Details on test material:

Charge No.: 110789
Purity: >99.0%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

- Preparation of test media: The test medium of the highest test concentration, 500 mg/l (nominal) was prepared together with 100 mg Cremophor RH 4 solubilizer. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. Additionally, a culture medium control and a solubilizer control was tested in parallel.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 hours
- Feeding during test
- Food type: grown Green algae culture
- Frequency: daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 +/- 0.5 mmol/l

Test temperature:

19 - 21 °C

pH:

7.6 - 8.1

Dissolved oxygen:

> 6 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 15.625, 31.3, 62.5, 125, 250, 500 mg/l. Additionally, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: flat bottom glass tubes; 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water chemistry according to the test guideline DIN 38412, L 11
- Total hardness: 2.70 +/- 0.5 mmol/l
- Acid capacity: 0.90 +- 0.1 mmol/l
- Ca/mg ratio: 4 :1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 µE/m²*s) at 400 - 750 nm wave length
- Observation time: 0, 3, 6, 24, 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

133.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Nominal 	Number of swimming animals after
conc.	
(mg/l) 		0h  3h  6h  24h 48h	
-------------------------------------
15.625 		20  20  20  20  20  
31.3 		20  20  20  20  20  
62.5 		20  19  19  19  19  
125.0 		20  18  17  16  14  
250.0 		20  13  0   0   0   
500.0 	 	20  0   0   0   0   
0		20  20  20  20  20  
Lk	 	20  20  20  20  20  
-------------------------------------

EC50 (24h): 148.24 mg/l (CI 128.57-170.9)
EC50 (48h): 133.57 mg/l (CI 112.56-158.49)
EC0 (48h): 62.5 mg/l
EC100 (48h): 250 mg/l

Remark: At test concentrations of 250 and 500 mg/l a yellow deposit was observed.