Cyclohexanone, peroxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A OECD GLP 203 guideline study, but with standards from 1990. Analytical measurment of substance concentration not included, and there is no certificate of analyses.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Poecilia reticulata

Details on test organisms:

The guppies were obtained from a aquarium retailer and were hold in stock for 10 days at the Research test lab. The size of the fish were approximatly 2 cm.

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

The diluting water was a synthetic water, Dutch Standard Water according to NPR 6507 (1980), having a ph of approximately 8.2 and a hardness of 13 dH, containing per litre of deionized water:
100 mg NaHCO3/L
20 mg KHCO3/L
200 mg CaCl2*H20/L
180 mg MgSO4*7H20

The deonized water contained not more than 0.01 mg copper per litre and had a conductivity of less than 5 µS/cm.

Test temperature:

24.5 - 25.5°C

pH:

8.2

Dissolved oxygen:

6.5-7,7 mgO2/L

Nominal and measured concentrations:

Only nominal values corrected for active ingredients are reported in this study.

Details on test conditions:

TEST SYSTEM
- Test vessel: 2Liters glass aquaria which contained 1 liter of test medium
- Renewal rate of test solution (frequency/flow rate): Semistatic test renewal ater 48 hours.
- No. of organisms per vessel: 10. In one of the test concentrations, one fish was unintentionally killed during removal of the test medium on day 2, and therefore the LC50 values for that concentration (18.9 mg/L) was calculated with 9 fish from they 0.
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates) 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dutch Standard Water
- Total organic carbon: The TOC-content was not more than 2.0 mg/L.
- Conductivity:less than 5 µS/cm.
- Intervals of water quality measurement: 48 hours


TEST CONCENTRATIONS

- Range finding study: The selected range of the test concentrations were determined in two preliminary range finding tests with 10-100 mg/L and 4.2 - 9.0 - 18.9 - 41.4 mg/L
- Test concentrations: 18.9 -41.4 and 90 mg/L

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

47.7 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95 % CL: 17.3 -131.6 mg/L

Reported statistics and error estimates:

In one of the test concentrations, one fish was unintentionally killed during removal of the test medium on day 2, and therefore the LC50 values for that concentration (18.9 mg/L) was calculated with 9 fish from they 0.

Validity criteria fulfilled:

yes

Remarks:

Mortality in control <10%, the oxygen concentration 60% of air saturation.

Executive summary:

This OECD 203 study performed under GLP conditions resulted in a LC50 of cyclohexanone peroxide to Guppy of 47.7 mg/L.The study meets the relevant validation criteria.

Description of key information

A OECD 203 GLP study was performed on hte cycloheaxonen peroxide. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

47.7 mg/L

Additional information

Data is availble for one OECD 203 study performed under GLP conditions. This resulted in a LC50 of cyclohexanone peroxide to Guppy of 47.7 mg/L. The study meets the relevant validation criteria.