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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted on read-across material
Remarks:
Study conducted to basic scientific principles, although only useful as supporting information as the result is an outlier compared to the weight of evidence from other results. The read-across from MnSO4 to Mn(NO3)2 is justified on the following basis: both substances are very soluble in water hence bioavailable and both will release Mn2+ ions. Therefore, from an ecotoxicity standpoint, the nitrate or sulphate anions are not considered to have any influence on the effective toxicity of Mn2+ or any toxicity in their own right, so the anions can be disregarded. Therefore any effect will be related to the Mn2+ cation, and the data from MnSO4 ecotoxicity tests is regarded as a suitable surrogate for read-across
Justification for type of information:
See the read-across report attached in Section 13.
Cross-reference
Reason / purpose for cross-reference:
other: Read-across target
Reference
Endpoint:
short-term toxicity to fish
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study conducted on read-across material
Justification for type of information:
See the read-across report attached in Section 13.
Reason / purpose for cross-reference:
read-across source
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
49.9 mg/L
Conc. based on:
other: Mn
Basis for effect:
mortality (fish)

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity of manganese to brown trout (salmo trutta) in hard water
Author:
Davies H & Brinkman S
Year:
1995
Bibliographic source:
Federal aid Project #F-243, Colorado Division of Wildlife, Fort Collins, Colorado

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A flow-through toxicity test using a modified Mount and Brungs diluter which delivered two litres of each concentration per cycle to 92 litre aquaria.Median lethal concentration (LC50) was determined using probit (Finney 1971) and Spearman-Karber (Hamilton et al. 1977 and 1978) methods
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Manganese sulphate monohydrate
IUPAC Name:
Manganese sulphate monohydrate

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Salmo trutta

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
49.9 mg/L
Conc. based on:
other: Mn
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Manganese concentrations were close to nominal values and were constant throughout the experiment. The LC50 in hard water (49.9mg Mn/L) is considerably higher than that for brown trout in much softer water (3.8 mg Mn/l) (Davies and Brinkrnan 1994). Therefore the difference between LC50s is due primarily to differences in water quality characteristics.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 was 49.9 mg Mn/L.The read-across from MnSO4 to Mn(NO3)2 is justified on the following basis: both substances are very soluble in water hence bioavailable and both will release Mn2+ ions. Therefore, from an ecotoxicity standpoint, the nitrate or sulphate anions are not considered to have any influence on the effective toxicity of Mn2+ or any toxicity in their own right, so the anions can be disregarded. Therefore any effect will be related to the Mn2+ cation, and the data from MnSO4 ecotoxicity tests is regarded as a suitable surrogate for read-across