Registration Dossier
Registration Dossier
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EC number: 231-274-1 | CAS number: 7474-78-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study under GLP-Conditions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,4-diaminobenzenesulphonic acid
- EC Number:
- 231-274-1
- EC Name:
- 3,4-diaminobenzenesulphonic acid
- Cas Number:
- 7474-78-4
- Molecular formula:
- C6H8N2O3S
- IUPAC Name:
- 3,4-diaminobenzene-1-sulfonic acid
- Details on test material:
- - Name of test material (as cited in study report): Orthaminsäure
- Physical state: solid
- Analytical purity: 99.1%
- Impurities (identity and concentrations): see 1.4
- Composition of test material, percentage of components: see 1.4
- Purity test date: 15.09.2011
- Lot/batch No.: N-18326
- Expiration date of the lot/batch: 15.09.2013
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge plant of Pforzheim
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2.42
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
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