Germanium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

see section 13 of IUCLID for read across justification

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations:0-6-12-24-48-96
- Sampling method:samples were taken at the start and the end of the test. 1 replica sample was taken of each test concentration. The samples were placed in PE bottles and 1% HNO3 was added to each sample.
- Sample storage conditions before analysis:room temperature before shipping

Vehicle:

no

Details on test solutions:

OECD guideline 203

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Danio rerio
- Source:De Siervis
- Length at study initiation (length definition, mean, range and SD):3.48 +- 0.04 cm
- Weight at study initiation (mean and range, SD):0.429 +- 0.042
- Feeding during test:no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

according to OECD 203

Test temperature:

22.2-26.6°C

pH:

8.04-8.56

Dissolved oxygen:

7.49 mg O2/L

Nominal and measured concentrations:

nominal concentrations: 0-6-12-24-48-96 mg geO2/L
measured concentrations: 0-6.47-12.95-25.05-50.35-103.5 mg geO2/L

Details on test conditions:

TEST SYSTEM
- Test vessel:3L borosilicate glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume:3L borosilicate glass beakers
- No. of organisms per vessel:7
- Biomass loading rate:7 fish per 3L corresponds with 1g fish /L


OTHER TEST CONDITIONS
- Photoperiod:14h daily photoperiod
- Light intensity:Philips TL-33 lamps yielding at the surface of the test solutions

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:2
- Range finding study:yes
- Test concentrations:0-0.2-0.58-1.81-5.76-18.1 mg Ge02/L
- Results used to determine the conditions for the definitive study:no mortality was observed in the range finding study

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 96 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: unbound NOEC

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

103.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 103.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: unbound NOEC

Validity criteria fulfilled:

yes

Conclusions:

Tests done according to standard protocol. Good quality and considered useful for setting the reference value for acute aquatic ecotoxicity.

Executive summary:

Tests done according to standard protocol.

The 96 h LC50 was >103.5 mg GeO2/L, or >71.8 mg Ge/L.

Description of key information

One study result of an OECD standard test was available on the short-term toxicity to fish. This study was conducted with GeO2 according to standard protocol.
The 96hrs LC50 was 103.5mg GeO2/L.

No studies on marine fish species were available.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

72 mg/L

Additional information

The 96h LC50 was 103.5 mg GeO2/L. This corresponds to 72 mg Ge/L