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EC number: 230-512-1 | CAS number: 7158-25-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Definitive (main) test: 30 July 2019 (Experimental start) to 1 August 2019 (Experimental completion)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3a,4,4a,5,8,8a,9,9a-octahydro-4,9:5,8-dimethano-1H-benz[f]indene
- EC Number:
- 230-512-1
- EC Name:
- 3a,4,4a,5,8,8a,9,9a-octahydro-4,9:5,8-dimethano-1H-benz[f]indene
- Cas Number:
- 7158-25-0
- Molecular formula:
- C15H18
- IUPAC Name:
- 3a,4,4a,5,8,8a,9,9a-octahydro-4,9:5,8-dimethano-1H-benz[f]indene
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source of test material: Materia, Inc.
- Lot/batch No.of test material: RP229-0714
- Expiration date of the lot/batch: 26 November 2019
- Purity test date: 26 November 2018 (CoA)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature (ambient, 15ºC ± 10ºC), in the dark
- Stability under test conditions: Assumed stable for the duration of the study
- Solubility and stability of the test substance in the solvent/vehicle: The test item was insoluble in the test media and therefore dosed with a carrier solvent, Dichloromethane (DCM). Assumed stable for the duration of the study.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: None
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Prepared by the direct addition of test item in solvent stock solutions
FORM AS APPLIED IN THE TEST (if different from that of starting material) : Prepared by the direct addition of test item in solvent stock solutions
OTHER SPECIFICS:
- measurement of pH and precipitate in the culture medium to which the test chemical is added:
Definitive test: Initial pH at 0 Hours: 7.36 (adjusted to 7.45) (Required: 6-9). pH range in control and test concentrations throughout test: 7.46 – 7.76.
No precipitate present as test item solutions were prepared by the direct addition of test item in solvent stock solutions.
- other information:
Initial Dissolved Oxygen (DO) at 0 Hours: 8.10mg/l, DO range in control and test concentrations throughout the test: 8.05 – 9.16mg/l (Required: ≥3mg/l)
Temperature range within incubator throughout test: 18.9 –19.8°C (Required: 20±2°C maintained to ±1°C).
Illumination: 16 hours - Light, 8 hours - dark cycle
Water quality measurements: The pH (to 0.01), dissolved oxygen mg/l (to 0.01) and temperature (to 0.1°C) measurements were taken at 0 hours on remaining test solution, and at 48-hour endpoint on pooled replicates for each test and control solution. Temperature of incubation was recorded at 0, 24 and 48 hours.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The test was carried out at concentrations of 0.02, 0.08, 0.32, 1.28 and 5.12mg/l. Additional sacrificial test vessels were prepared without Daphnia. These solutions were extracted and analysed to verify the initial concentrations of Tricyclopentadiene (Distilled) in the test at 0hrs. Once extracted, the samples were concentrated by a factor of 10 to final concentrations of 0.2, 0.8, 3.2, 12.8 and 51.2 mg/l prior to analysis.
- Sampling method: A single method of analysis using High Performance Liquid Chromatography (HPLC) was used to determine the measured concentration of Tricyclopentadiene (Distilled) in the toxicity test exposure solutions.
- Sample storage conditions before analysis: Analytical samples unable to be processed within an acceptable time-frame were frozen and stored immediately after collection until use.
Test solutions
- Vehicle:
- yes
- Remarks:
- TCPD was insoluble in the test media and therefore dosed with a carrier solvent, Dichloromethane (DCM).
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Information provided by the Sponsor indicated that the sample was insoluble in water. Therefore, both the prelim and the definitive test were prepared by the direct addition of test item in solvent stock solutions.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Originally sourced from a clone of D. magna genotype 5
- Justification for species other than prescribed by test guideline: None, species used as per OECD 202 guideline
- Age at study initiation (mean and range, SD): ≤ 24 hours.
- Stage and instar at study initiation: Not specified
- Source: Actual source prior to arrival at Chemex (in 2014) not specified.
- Feeding during test : Daphnids were not fed during the test period.
- Food type: Each working day, Daphnia were fed a suspension of Unicellular green algae (Chlorella vulgaris)
- Amount: Not specified
- Frequency: Each working day
ACCLIMATION
- Acclimation period: Not specified
- Acclimation conditions (same as test or not): Same
- Type and amount of food: Daphnia were fed a suspension of Unicellular green algae (Chlorella vulgaris), quantity not specified
- Feeding frequency: Each working day
- Health during acclimation (any mortality observed): All Daphnia were healthy, no mortality observed.
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS: Not specified
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- The objective was determine the 48-hour EC50 according to OECD test guideline 202.
Test conditions
- Test temperature:
- Temperature: 20 ± 2°C. Temperature range within incubator throughout test: 18.9 – 19.8°C
- pH:
- Stabilised between pH 6 and 9 before commencement of the test. pH range in control and test concentrations throughout test: 7.46 – 7.76
- Dissolved oxygen:
- Dissolved oxygen: >60% (or 3mg/l) Absolute Saturation Volume. Initial Dissolved Oxygen at 0 Hours: 8.10mg/l.
DO range in control and test concentrations throughout test: 8.05 – 9.16mg/l (Required: ≥3mg/l) - Nominal and measured concentrations:
- All concentrations of the test substance are reported as nominal as received. As Tricyclopentadiene (Distilled) was insoluble in test media it was therefore dosed with a carrier solvent, Dichloromethane (DCM).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 40ml glass dishes.
- Type (delete if not applicable): Open, however to limit aerial contamination and reduce evaporative losses, the vessels were covered with a perspex sheet.
- Material (Glass dishes), size (40 mL)l, fill volume (40 mL)
- Volume of solution: 30 mL (Dose Range Finding Test), 40 mL (Definitive test)
- Aeration: Not specified, but oxygen saturation above accepted guideline parameters
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per solvent control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: De-ionised water
- Culture medium different from test medium: No, Daphnia were cultured, and the tests performed, in Elendt M4 reconstituted Daphnia water.
- Intervals of water quality measurement: Not specified
OTHER TEST CONDITIONS
- Adjustment of pH: Initial pH at 0 Hours: 7.36 (adjusted to 7.45) (Required: 6-9). pH range in control and test concentrations during test: 7.46 – 7.76
- Photoperiod: : 16 hours - light, 8 hours - dark cycle
- Light intensity: Not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The number of immobilised Daphnia were recorded after 24 and 48-hour exposure periods.
SOLVENT CONTROL PERFORMED: Yes , all test substance concentrations are reported as nominal as received. TCPD was insoluble in the test media and therefore dosed with a carrier solvent Dichloromethane (DCM).
RANGE-FINDING STUDY
- Test concentrations: (0) Control, Solvent control, 0.01, 0.10, 1.0 and 10 mg/L TCPD
- Results used to determine the conditions for the definitive study: Data from the preliminary test identified the 48-hour EC50 as being between 0.1-1.0mg/l. The concentrations selected for the definitive study were: (0) Control, Solvent control, 0.02, 0.08, 0.32, 1.28 and 5.12 mg/l. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.321 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities:
Daphnia were examined at 24 and 48-hours for abnormal behaviour during the determination of immobility. Abnormalities were observed during the test period.
- Other biological observations: Daphnia trapped in meniscus (mobile), Daphnia trapped in meniscus (immobile)
- Mortality of control: None
- Other adverse effects control: None
- Immobilisation of control: One Daphnia imobilised at 24 and 48 hours
- Immobilisation of solvent control: One Daphnia immobilised at 24 hours, and two at 48 hours.
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No, all test solutions prepared by direct addition of test item in solvent stock solutions.
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, a separate GLP study was conducted (June 2019) to monitor the sensitivity of the test animals and test procedures. The reference study gave a 24 hour EC50 of 1.07mg/l of potassium dichromate which was within the acceptable range 0.6 mg/l to 2.1 mg/l as specified in Technical Corrigendum to ISO6341.
- Reported statistics and error estimates:
- Where possible, the EC10 and EC50 values with 95% confidence limits and NOEC were calculated according to the statistical methods of ToxCalc™ Version 5.0 “Comprehensive Toxicity Data Analysis and Database Software”, copyright 1994-1996.
The 24-hour EC50 and 48-hour EC50 of Tricyclopentadiene (Distilled) to Daphnia magna were 1.679mg/l (determined by Maximum Likelihood Probit (P = 0.11), using ToxCalc v5.0) and 1.321mg/l (Determined by Determined by Maximum Likelihood Probit (P = 0.38), using ToxCalc v5.0), respectively.
The 0 to 24-hour NOEC and LOEC were 0.08mg/l and 0.32mg/l respectively (determined by Bonferroni t-test with Arcsin square root transformation, 1-tail, P=0.05, following tests for normality of distribution and equal variances, using ToxCalc v5.0)
Any other information on results incl. tables
Cumulative immobilisation
Nominal Conc (mg/l) |
No. immobilised |
No. Immobilised |
||
|
24 hours |
48 hours |
24 hours |
48 hours |
0 (Control) |
1 |
1 |
5 |
5 |
Solvent control |
1 |
1 |
5 |
10 |
0.02 |
2 |
2 |
10 |
10 |
0.08 |
1 |
1 |
5 |
5 |
0.32 |
10 |
9 |
50 |
45 |
1.28 |
10 |
11 |
50 |
55 |
5.12 |
12 |
12 |
60 |
70 |
Observations
Nominal Conc (mg/l) |
Recorded observations |
|
|
24 hours |
48 hours |
0 (Control) |
19NS 19NS |
19NS 19NS |
Solvent control |
18NS, 1TM |
18NS |
0.02 |
18NS |
17NS, 1TM |
0.08 |
14NS, 5TM |
15NS, 4TI |
0.32 |
6NS, 4TM |
9NS, 2TI |
1.28 |
6NS, 3TM, 1TI |
6NS, 3TM |
5.12 |
4NS, 4TM |
4NS, 1TM, 1TI |
Key:
S |
Sedimentation |
Ms |
Movement Slower |
D |
Debris attached to Daphnia |
TM |
Trapped in Meniscus (mobile) |
LI |
Lighter Colouration |
TI |
Trapped in Meniscus (immobile) |
NS |
No Signs of Abnormality |
SM |
DaphniaSmaller |
- |
All Daphnia immobilised |
|
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The definitive test (conducted 30th July to 1st August 2019) was performed according to OECD guideline 202 (2004) and met all validity criteria.
- Conclusions:
- The definitive test conducted from 30th July to 1st August 2019 was performed according to OECD guideline 202 (2004) and met all validity criteria.
The 48-hour EC50 of Tricyclopentadiene (Distilled) to Daphnia magna was 1.321mg/l (determined by Maximum likelihood-probit).
The 0 to 48-hour NOEC and LOEC were 0.08mg/l and 0.32mg/l respectively (determined by Dunnett’s Test with Arcsin square root transformation).
Analytical confirmation of nominal test concentrations by HPLC was performed.
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