3-hydroxy-4-[(2-methyl-5-nitrophenyl)azo]-N-phenylnaphthalene-2-carboxamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

From 02.OCT 1981 to 09. OCT 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed similar to OECD 203, sufficiently well documented, pre-GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-(4-chloro-2,5-dimethoxyphenyl)-3-hydroxy-4-[[2-methoxy-5-[(phenylamino)carbonyl]phenyl]azo]naphthalene-2-carboxamide

EC Number:

226-103-2

EC Name:

N-(4-chloro-2,5-dimethoxyphenyl)-3-hydroxy-4-[[2-methoxy-5-[(phenylamino)carbonyl]phenyl]azo]naphthalene-2-carboxamide

Cas Number:

5280-68-2

IUPAC Name:

N-(4-chloro-2,5-dimethoxyphenyl)-3-hydroxy-4-{[2-methoxy-5-(phenylcarbamoyl)phenyl]diazenyl}-2-naphthamide

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution of the test item was prepared in test water, applied to the test tanks and evenly distributed by a glass rod.

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Breeder: Paul Eggers, 2345 Hohenwestedt
Acclimation: Fish were kept at least for 14 days in test water at 20 deg C before start of the experiment.
Body weight: 1.8 - 2.5 g (arithmetic mean: 2.1 g; sd = 0.26 g; n = 10)
Body length: 5.5 - 6.7 cm (arithmetic mean: 6.3 cm; sd = 0.34 cm; n = 10)
Corpulence factor: 0.8 - 1.08 (arithmetic mean: 0.86; sd = 0.09; n = 10)
Feeding: Tetra Min (Tetra Werke, Melle)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Test conditions

Hardness:

Total hardness: 9.5 deg dH
Carbonate hardness: 6.4 deg dH

Test temperature:

20 +/- 1 deg C

pH:

7.8 - 8.2

Dissolved oxygen:

> 7 mg/L (7.0 - 8.0 mg/L)

Nominal and measured concentrations:

0, 1, 10, 100, 500 mg/L nominal concentrations

Details on test conditions:

For each concentration group and the control 10 fish were used.
Deionized tap water ( < 5 µS/cm) was supplemted with the following salts and used as test water:
192 mg/L NaHCO3
120 mg/L CaSO4*2H2O
120 mg/L MgSo4
8 mg/L KCl

Test basins made of glass were used (40 x 25 x 30 cm) and filled with 20 L test water. The test temperature of 20 +/- 1 deg C was kept constant by means of a water bath. Test tanks were aerated during the whole test period by glass capillaries with a aeration rate of 100 mL air/min. Day-night regime was 12 hours, illumination strength approximately 700 Lux.

Approximately 65 hours preceding the experiment 10 fish, each were introduced into the test basins (20L).
Immediately preceding test item application and after 2, 24, 48, 72 and 96 hours pH, O2-concentration and temperature were determined.

During the whole experiment fish were observed on a regular basis and their behaviour was recorded.
At the end of the incubation period fish were killed in 0.05% 3-Aminobenzoic acid ethyl ester methanesulfonate, dissected and macroscopically examined.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

containing 40% submission substance, i.e. LC50 > 200 mg/L submission substance

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

containing 40% submission substance, i.e. EC50 > 200 mg/L submission substance

Basis for effect:

behaviour

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

containing 40% submission substance, i.e. LC0 = 200 mg/L submission substance

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

containing 40% submission substance, i.e. EC0 = 200 mg/L submission substance

Basis for effect:

behaviour

Details on results:

Neither for the control nor for the treatment groups any dead fish were observed. No differences in behaviour were observed in the treatment groups compared to the control group. The onl y macroscopic observation made was a slightly pink colouring of the fins at the highest concentration (500 mg/L test material).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test on the fish species Leuciscus idus similar to OECD 203 the submission substance proved to have no toxic or behavioural effects up to the highest tested concentration of 200 mg/L submission substance (500 mg/L test material).
LC50 (96h) > 200 mg/L (>500 mg/L test material)
LC0 (96h) = 200 mg/L (= 500 mg/L test material).

Executive summary:

In an acute toxicity test on the fish species Leuciscus idus similar to OECD 203 (reliability category 2) the submission substance proved to have no toxic or behavioural effects up to the highest tested concentration of 200 mg/L submission substance (500 mg/L test material). In the test material, the submission substance was present as a stable dispersion miscible with water.

LC50 (96h) > 200 mg/L (>500 mg/L test material)

LC0 (96h) = 200 mg/L (= 500 mg/L test material).