Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

EC Number:

228-726-5

EC Name:

Tetraethyl 2,2'-(1,4-phenylenedimethylidyne)bismalonate

Cas Number:

6337-43-5

Molecular formula:

C22H26O8

IUPAC Name:

1,3-diethyl 2-({4-[3-ethoxy-2-(ethoxycarbonyl)-3-oxoprop-1-en-1-yl]phenyl}methylidene)propanedioate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, adapted

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

5

Sampling time:

28 d

Remarks on result:

other: mean biodegradation

Details on results:

The biochemical oxygen demand (BOD) of the test substance in the test media very slowly increased throughout the exposure period. At the end of the 28-day exposure period, the mean biodegradation of the test item amounted to 5 %. Consequently, the test item was found not biodegradable under the test conditions within 28 days because biodegradation was below 10 %.

Any other information on results incl. tables

The percent biodegradation of the test item was calculated based on the theoretical oxygen demand (ThOD) of 1.88 mg O2/mg test item.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test item was found not biodegradable under the test conditions within 28 days because biodegradation was below 10 %.

Executive summary:

The test item was investigated for its ready biodegradability in a manometric respirometry test over 28 days according to EU Commission Directive 92/69 EEC, C.4 -D (1992) and OECD Guideline for Testing of Chemicals No. 301 F (1992). The percent biodegradation of the test item was calculated based on the theoretical oxygen demand (ThOD) of 1.88 mg O2/mg test item.

The biochemical oxygen demand (BOD) of the test item in the test media very slowly increased throughout the exposure period. At the end of the 28 -day exposure period, the mean biodegradation of the test substance amounted to 5 %. Consequently, the test item was found not biodegradable under the test conditions within 28 days because biodegradation was below 10 %. No degradation of the test item occurred in the abiotic control under the test conditions within 28 days.