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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline conform study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 1984-04-04
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylene diacetoacetate
EC Number:
226-724-9
EC Name:
Ethylene diacetoacetate
Cas Number:
5459-04-1
Molecular formula:
C10H14O6
IUPAC Name:
ethane-1,2-diyl bis(3-oxobutanoate)

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
At the beginning and after 48 and 96 hours samples were taken from the bassins containing 220 mg/L and 1000 mg/L of the test substance. The content of the test item was then determined by HPLC.
theoretical concentration : 220 mg/L; found (average) 220.7 mg/L
1000 mg/L; found (average) 1005 mg/L

Test solutions

Vehicle:
no
Details on test solutions:
Water with a composition according ISO/DIS 7346/1 was used

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Type: Hamilton-Buchanan
Source: West Aquarium, 3422 Bad Lauterberg
born: 10.10.1988 / 02.12.1988
delivered: 17.01.1989 / 07.02.1989

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.6 - 22.9 °C
pH:
7.1 - 7.8
Dissolved oxygen:
5.0 - 9.0
Nominal and measured concentrations:
nominal 220 mg/L measured: 220.7 mg/L
nominal 1000 mg/L, measured 1005 mg/L
Details on test conditions:
Test was performed in two steps:
part 1: concentrations: 0, 500 mg/L
part 2: concentrations: 0, 220, 1000 mg/L
10 fish per concentration
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
220 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: LC30
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item is not subject for classification for acute toxicity to fish since LD50 is between 500 and 1000 mg/L
Executive summary:

Ethylene diacetoacetat was tested for toxicity to fish according OECD guideline 203. The mortality was 0% at 220 mg/L, 30% at 500 mg/l and 100% at 1000 mg/L after 96 hours. The LD50 is therefore between 500 and 1000 mg/L.